FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n01_AP3-F02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n01_AP3-F02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76792
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGAACT150.002216980370.070
ATACCCA150.002216980370.06
GTCAGCG200.006927468352.4999969
ACTCGGT200.006927468352.49999612
TACTACA200.006927468352.4999964
CCCACTA450.00428369531.1111131
GAGAAGT500.007169173628.0000026
CTAGGCA952.7487875E-422.10526370
ACTAGGC1055.4030016E-420.069
TTCCTTG900.005233007519.4444453
GACTAGG1159.963983E-418.2608768
GTTTTCA1000.00961065117.50000211
AGACTAG1300.002264734116.15384767
GAGACTA1450.00468667714.482758566
TCCGAGC4753.6411184E-69.57894770
CTCCGAG5006.991473E-69.09999969
TCTCCGA5552.6082096E-58.19819868
ATCTCCG6251.1448846E-47.2867
CATCTCC7004.5961348E-46.566
ACATCTC7500.00105919696.06666665
Produced by FastQC (version 0.11.5)