FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n01_AP3-E11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n01_AP3-E11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57678
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTAG150.002214170870.0000164
GACCGTC252.3719718E-455.9999967
ACTCACT200.006918756352.511
CTTGGTC350.001245874840.0000043
AAAACTG400.002400486335.032
CCTGGAT400.002400486335.034
AATAGTG552.9585746E-431.818185
CATATAC500.007154588627.9999983
ATATACA900.00521750119.4444454
TCTTATA2254.6287762E-412.44444570
CTCTTAT2750.002481758410.18181869
TCTCTTA3000.00509747169.33333468
GTCTCTT3050.00584069039.18032767