FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n01_AP3-E09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n01_AP3-E09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36199
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATTT150.002207466470.070
ACCGATT150.002207466470.069
TTATCGC150.002207466470.062
TGACATG200.00689796752.56
CTAAAAC200.00689796752.53
TATAGCG200.00689796752.55
TATACAG200.00689796752.55
GCAAGTA350.001239707240.027
GAAAGAA604.890562E-429.16666668
TTATTCT500.007119804627.9999984
AGAATAT500.007119804627.99999820
ATATACT657.810405E-426.9230778
ATATACA800.002617984521.8754
TATATAT1604.992448E-415.31253
ATATATA1550.007194440313.5483872
Produced by FastQC (version 0.11.5)