FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n01_AP3-E06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n01_AP3-E06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31593
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCAT150.002204848770.027
CTCCGAA150.002204848770.012
TCACACA150.002204848770.030
GCAGGCT150.002204848770.054
CGCACCT150.002204848770.066
CCGCACC150.002204848770.065
TCCGCAC200.00688984852.564
ATCAGCC200.00688984852.554
ATCACAC200.00688984852.529
GCACCTT200.00688984852.567
AGATTCC200.00688984852.549
GTATAGC200.00688984852.51
ATACAGT200.00688984852.54
GATACGA305.79242E-446.66666415
CAAAATC305.79242E-446.66666450
CTATGCA350.001237301340.09
TAATACT400.00238406635.04
AAATCAG400.00238406635.052
ATATAAT501.6731457E-435.01
ATACGAA500.007106237627.99999816
Produced by FastQC (version 0.11.5)