FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n01_AP3-D05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n01_AP3-D05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60388
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGAACC150.00221467670.04
GTATGAA150.00221467670.09
AGACCTC150.00221467670.040
CAGTGGA150.00221467670.09
TCCAGAG200.006920322352.52
AACCTAA200.006920322352.525
TAAACCT400.002401376635.04
GCGTTTC400.002401376635.013
TATACTG400.002401376635.05
CCTTTAT450.00427650231.1111091
GACAAAA500.00715720827.99999833
TCCGAGC1350.00290355915.55555570
CTCCGAG1400.0036993215.069
ATCTCCG1550.007268558713.54838767
TCTCCGA1550.007268558713.54838768
ATACACA2800.00288530210.070
TATACAC2900.00385785579.65517269
Produced by FastQC (version 0.11.5)