FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n01_AP3-A11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n01_AP3-A11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64488
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGCAG150.00221536270.045
CTATAGA200.006922449552.54
TCTATCC200.006922449552.53
TCCTATA556.6013854E-638.181822
ATCCTAT703.440516E-530.01
CCACGAG851.290387E-424.70588370
TATTCTT952.7421524E-422.1052635
TTATTCT800.00264019221.8754
CCCACGA1055.3900713E-420.069
GCCCACG1107.3729496E-419.0909168
TCCTGTC1107.3729496E-419.0909162
TCTTCCT1000.00959440417.50000258
AGCCCAC1400.003702967415.067
CGAGCCC1550.0072756313.54838765
GAGCCCA1600.00897475313.12566
CCTGTCT3702.4134215E-510.40540563
CTTATAC5754.3760338E-79.13043570
TCTTATA6101.0268195E-68.60655869
CTCTTAT6402.0448842E-68.20312568
TCTCTTA7008.457082E-78.067
Produced by FastQC (version 0.11.5)