FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n01_AP2-E01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n01_AP2-E01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48508
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGTTC150.002212043470.054
GAGGTTC150.002212043470.07
AGTCTAT150.002212043470.036
CAGACCT150.002212043470.04
CGCGTTT200.00691215852.517
TTATAGA350.001243916140.04
AACTGGG350.001243916140.066
TGGACAG450.004268286331.1111095
TATTATA500.00714354227.9999982
CTATTAG500.00714354227.9999981
ATATATA1001.3631987E-524.4999981
CCGAGCC1205.5744604E-520.41666670
GAGCCCA900.005205767719.44444369
TCCGAGC1301.0313386E-418.84615569
CTCCGAG1452.3793464E-416.89655168
TCTCCGA1707.977865E-414.41176567
ATCTCCG1850.001511292813.24324366
CATCTCC2050.003267810411.9512265
ACATCTC2100.003913318711.66666764