FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n01_AP2-D10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n01_AP2-D10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40365
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTCTA150.002209336570.010
CTGATGG200.006903765752.551
TGCAAAG200.006903765752.531
AGGAATT350.001241426740.061
CCACGAG350.001241426740.070
TATACTT552.9429258E-431.818185
GCCCACG450.004259845731.11111368
AGAAGTT450.004259845731.1111135
AGCCCAC500.007129528.00000267
CCCACGA500.007129528.00000269
Produced by FastQC (version 0.11.5)