FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n01_AP2-D01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n01_AP2-D01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79451
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGAT200.00692836252.53
TAAGGGG200.00692836252.54
TAGGATT350.001248727640.0000044
TTAGACA450.004284595631.1111134
CTAAGGG450.004284595631.1111133
TCTAAGG450.004284595631.1111132
TTATACT500.00717067327.9999984
GAATAAG700.001216530125.0000021
CAGGTGA750.001818124323.33333455
ATATAAA1055.4053054E-420.01
ATTGAAC1000.00961354817.53
CTCCGAG2750.0024995910.18181870
TCTCCGA2800.00290257810.00000169
CATCTCC3150.00766636158.88888967
Produced by FastQC (version 0.11.5)