FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n01_AP1-F01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n01_AP1-F01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92874
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGAT150.002218451670.031
GTCCTAG200.00693202752.50000444
TCCTAAG200.00693202752.5000042
GCCGATG200.00693202752.50000432
TATACGG400.00240803635.0000045
GGGCTTT450.00428828931.1111136
ATACGGT450.00428828931.1111136
ACGGTGT450.00428828931.1111138
TACGGTG500.007176818327.9999987
AATAGAG500.007176818327.9999985
TACACAA657.9040683E-426.9230775
CATATAC750.001820430223.3333343
GTTCCAT750.001820430223.3333346
GTATATA750.001820430223.3333341
GTTCAAT800.00264890421.8750021
TATATAG850.003764967420.5882365
TAGAATA950.0071620318.4210534
GAATATA950.0071620318.4210531
ATAGAAG950.0071620318.4210533
TATTACA950.0071620318.4210532
Produced by FastQC (version 0.11.5)