FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n01_AP1-E04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n01_AP1-E04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107645
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCT200.006935010752.58
CTCTCAG200.006935010752.528
AGCAGTC200.006935010752.57
GTATAGG459.146412E-538.8888851
CTGAGGA400.00240973435.068
CAAGTCA450.00429129531.11110929
AAGACAG500.00718181328.00000265
CAGAACA700.001219341625.09
CGAGACG700.001219341625.067
TAAGAGA700.001219341625.04
GAGACGG851.2982787E-424.70588368
AGACGGA750.001822305323.33333469
GACGGAC750.001822305323.33333470
ATATACT1102.8918055E-522.2727284
ACGAGAC850.003768807720.58823666
TGAATGA1000.00963509317.5000025
CACGAGA1250.001748622316.865
TAAAGAA1605.108301E-415.31255
TTTTGTT1800.00124634213.61111150
TATACAC3904.3832086E-59.87179470
Produced by FastQC (version 0.11.5)