FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n01_AP1-C04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n01_AP1-C04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107676
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTGT200.0069350252.520
ACTACAC350.001250706340.03
CATATAC552.9755943E-431.818183
TACTATG755.541843E-528.0000027
TATATAG500.007181830227.9999985
TCATATA906.1077226E-627.2222212
ATAATAG657.912315E-426.9230773
TACATAC657.912315E-426.9230773
TTCTACA808.599254E-526.252
TATAGAA700.001219345125.02
CCTTTAA800.002651627921.8751
TTAATCT850.003768819220.5882364
TATACTG850.003768819220.5882365
TATGATA900.005246470719.4444432
TTTATAC1107.417104E-419.0909083
GAATATA950.007169281618.4210511
ATCATAT1200.001330396917.51
TTTTATA1656.452444E-414.8484852
GCCCACG1550.0073175113.54838770
AGCCCAC1550.0073175113.54838769
Produced by FastQC (version 0.11.5)