FastQCFastQC Report
Wed 12 Dec 2018
H2NL2AFXY_n01_AP1-B07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2NL2AFXY_n01_AP1-B07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119222
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG305.860353E-446.6630361
CCTGGAA450.00429477331.1086864
CCTGATG450.00429477331.1086863
TTGGCCT500.00718760927.997824
AACCAAC500.00718760927.9978234
AAGTCCA500.00718760927.9978215
GAGACAG1558.586703E-720.32952570
CGAGACA1558.586703E-720.32952569
ACGAGAC1651.5637142E-619.09743368
GTAATAA950.00717691518.4196191
CTACATC950.00717691518.4196194
CACGAGA2051.2362778E-515.37110567
CCACGAG2202.4101098E-514.32307366
CCCACGA2252.990659E-513.99890965
GCCCACG2354.5023713E-513.40321264
AGCCCAC2701.6492828E-411.66575863
CGAGCCC3451.1304615E-511.16323370
GAGCCCA2953.7494226E-410.677134562
CCGAGCC3652.1099948E-510.55154969
CTCCGAG4005.7789694E-59.62828867
Produced by FastQC (version 0.11.5)