MultiQC Report

A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

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Report generated on 2025-01-30, 18:47 based on data in: /scratch/gencore/GENEFLOW/work/nf/6b/e996b69c82adb5d07100cd78759032/3

General Statistics

Showing ³⁸⁶/₃₈₆ rows and ³/₅ columns.

Sample Name	% Dups	% GC	Length	% Failed	M Seqs
H2MF5DSXF_l03_n01_ECA211_POOLRET88	24.5%	40%	151 bp	18%	14.9
H2MF5DSXF_l03_n01_ECA3023_POOLRET88	22.8%	41%	151 bp	9%	17.0
H2MF5DSXF_l03_n01_ECA3089_POOLRET88	21.6%	41%	151 bp	9%	3.6
H2MF5DSXF_l03_n01_ECA3092_POOLRET88	21.9%	41%	151 bp	9%	13.2
H2MF5DSXF_l03_n01_ECA3094_POOLRET88	23.8%	41%	151 bp	9%	17.4
H2MF5DSXF_l03_n01_ECA3212_POOLRET88	21.9%	39%	151 bp	9%	14.8
H2MF5DSXF_l03_n01_ECA3222_POOLRET88	21.0%	39%	151 bp	9%	10.9
H2MF5DSXF_l03_n01_ECA3230_POOLRET88	23.2%	39%	151 bp	9%	13.8
H2MF5DSXF_l03_n01_ECA3240_POOLRET88	20.9%	38%	151 bp	9%	11.5
H2MF5DSXF_l03_n01_ECA3252_POOLRET88	22.3%	38%	151 bp	9%	13.7
H2MF5DSXF_l03_n01_ECA3256_POOLRET88	26.1%	38%	151 bp	9%	22.3
H2MF5DSXF_l03_n01_ECA3529_POOLRET88	23.1%	39%	151 bp	9%	15.7
H2MF5DSXF_l03_n01_ECA3530_POOLRET88	23.0%	39%	151 bp	9%	14.5
H2MF5DSXF_l03_n01_ECA3531_POOLRET88	21.3%	40%	151 bp	9%	14.7
H2MF5DSXF_l03_n01_ECA3532_POOLRET88	24.0%	39%	151 bp	9%	18.6
H2MF5DSXF_l03_n01_ECA3533_POOLRET88	22.5%	39%	151 bp	9%	14.6
H2MF5DSXF_l03_n01_ECA3534_POOLRET88	22.8%	38%	151 bp	9%	16.3
H2MF5DSXF_l03_n01_ECA3535_POOLRET88	23.5%	38%	151 bp	9%	17.4
H2MF5DSXF_l03_n01_ECA3536_POOLRET88	20.8%	38%	151 bp	9%	7.7
H2MF5DSXF_l03_n01_ECA3537_POOLRET88	21.5%	39%	151 bp	9%	14.9
H2MF5DSXF_l03_n01_ECA3538_POOLRET88	21.7%	39%	151 bp	18%	14.4
H2MF5DSXF_l03_n01_ECA3539_POOLRET88	21.5%	41%	151 bp	9%	9.6
H2MF5DSXF_l03_n01_ECA3540_POOLRET88	23.2%	40%	151 bp	9%	17.6
H2MF5DSXF_l03_n01_ECA3541_POOLRET88	19.7%	40%	151 bp	9%	14.3
H2MF5DSXF_l03_n01_ECA3543_POOLRET88	26.3%	38%	151 bp	18%	17.3
H2MF5DSXF_l03_n01_ECA3544_POOLRET88	22.6%	40%	151 bp	9%	12.8
H2MF5DSXF_l03_n01_ECA3545_POOLRET88	19.6%	41%	151 bp	9%	12.8
H2MF5DSXF_l03_n01_ECA3546_POOLRET88	20.8%	39%	151 bp	9%	13.4
H2MF5DSXF_l03_n01_ECA3548_POOLRET88	24.9%	39%	151 bp	9%	19.1
H2MF5DSXF_l03_n01_ECA3549_POOLRET88	22.5%	39%	151 bp	9%	15.6
H2MF5DSXF_l03_n01_ECA3550_POOLRET88	20.7%	38%	151 bp	18%	8.8
H2MF5DSXF_l03_n01_ECA3551_POOLRET88	22.9%	38%	151 bp	9%	17.1
H2MF5DSXF_l03_n01_ECA3552_POOLRET88	20.9%	40%	151 bp	9%	13.6
H2MF5DSXF_l03_n01_ECA3553_POOLRET88	24.5%	41%	151 bp	9%	20.4
H2MF5DSXF_l03_n01_ECA3554_POOLRET88	23.5%	41%	151 bp	9%	16.6
H2MF5DSXF_l03_n01_ECA3555_POOLRET88	21.8%	40%	151 bp	9%	14.2
H2MF5DSXF_l03_n01_ECA3556_POOLRET88	22.9%	39%	151 bp	9%	12.7
H2MF5DSXF_l03_n01_ECA3557_POOLRET88	22.7%	40%	151 bp	18%	16.2
H2MF5DSXF_l03_n01_ECA3558_POOLRET88	21.9%	38%	151 bp	9%	14.7
H2MF5DSXF_l03_n01_ECA3560_POOLRET88	21.4%	40%	151 bp	9%	14.2
H2MF5DSXF_l03_n01_ECA3561_POOLRET88	23.1%	39%	151 bp	9%	15.2
H2MF5DSXF_l03_n01_ECA3562_POOLRET88	22.4%	39%	151 bp	9%	14.8
H2MF5DSXF_l03_n01_ECA3563_POOLRET88	23.7%	40%	151 bp	9%	15.5
H2MF5DSXF_l03_n01_ECA3564_POOLRET88	22.3%	41%	151 bp	18%	14.5
H2MF5DSXF_l03_n01_ECA3567_POOLRET88	21.7%	40%	151 bp	9%	15.8
H2MF5DSXF_l03_n01_ECA3568_POOLRET88	23.0%	40%	151 bp	18%	15.2
H2MF5DSXF_l03_n01_ECA3569_POOLRET88	22.3%	40%	151 bp	9%	17.0
H2MF5DSXF_l03_n01_ECA3570_POOLRET88	23.9%	39%	151 bp	9%	14.9
H2MF5DSXF_l03_n01_ECA3608_POOLRET88	20.0%	39%	151 bp	9%	12.7
H2MF5DSXF_l03_n01_ECA3707_POOLRET88	24.2%	41%	151 bp	9%	17.5
H2MF5DSXF_l03_n01_ECA3708_POOLRET88	21.5%	40%	151 bp	9%	7.7
H2MF5DSXF_l03_n01_ECA3709_POOLRET88	21.5%	41%	151 bp	9%	15.0
H2MF5DSXF_l03_n01_ECA3710_POOLRET88	19.3%	42%	151 bp	9%	11.5
H2MF5DSXF_l03_n01_ECA3711_POOLRET88	22.8%	41%	151 bp	9%	15.8
H2MF5DSXF_l03_n01_ECA3712_POOLRET88	21.5%	41%	151 bp	9%	13.1
H2MF5DSXF_l03_n01_ECA3713_POOLRET88	21.1%	41%	151 bp	9%	12.7
H2MF5DSXF_l03_n01_ECA3889_POOLRET88	21.5%	42%	151 bp	9%	13.6
H2MF5DSXF_l03_n01_NIC757_POOLRET88	22.9%	41%	151 bp	9%	18.9
H2MF5DSXF_l03_n01_QG4629_POOLRET88	24.3%	41%	151 bp	9%	13.5
H2MF5DSXF_l03_n01_QG4630_POOLRET88	21.0%	40%	151 bp	9%	12.9
H2MF5DSXF_l03_n01_QG4637_POOLRET88	23.9%	40%	151 bp	18%	20.9
H2MF5DSXF_l03_n01_QG4641_POOLRET88	21.6%	40%	151 bp	9%	11.2
H2MF5DSXF_l03_n01_QG4646_POOLRET88	21.1%	41%	151 bp	9%	11.7
H2MF5DSXF_l03_n01_QG4647_POOLRET88	23.2%	41%	151 bp	9%	18.7
H2MF5DSXF_l03_n01_QG4648_POOLRET88	21.9%	40%	151 bp	9%	14.4
H2MF5DSXF_l03_n01_QG4653_POOLRET88	22.8%	41%	151 bp	9%	13.9
H2MF5DSXF_l03_n01_QG4659_POOLRET88	26.5%	40%	151 bp	18%	20.3
H2MF5DSXF_l03_n01_QG4661_POOLRET88	22.4%	41%	151 bp	9%	13.4
H2MF5DSXF_l03_n01_QG4669_POOLRET88	22.2%	41%	151 bp	9%	13.4
H2MF5DSXF_l03_n01_QG4671_POOLRET88	26.3%	40%	151 bp	18%	23.2
H2MF5DSXF_l03_n01_QG4672_POOLRET88	25.7%	40%	151 bp	9%	23.8
H2MF5DSXF_l03_n01_QG4681_POOLRET88	21.8%	40%	151 bp	9%	17.1
H2MF5DSXF_l03_n01_QG4682_POOLRET88	22.4%	40%	151 bp	9%	14.1
H2MF5DSXF_l03_n01_QG4683_POOLRET88	26.2%	41%	151 bp	9%	20.0
H2MF5DSXF_l03_n01_QG4694_POOLRET88	22.3%	40%	151 bp	9%	14.4
H2MF5DSXF_l03_n01_QG4695_POOLRET88	24.7%	41%	151 bp	18%	19.8
H2MF5DSXF_l03_n01_QG4696_POOLRET88	24.3%	41%	151 bp	9%	16.0
H2MF5DSXF_l03_n01_QG4698_POOLRET88	20.9%	40%	151 bp	9%	14.7
H2MF5DSXF_l03_n01_QG4700_POOLRET88	21.8%	41%	151 bp	9%	14.4
H2MF5DSXF_l03_n01_QG4701_POOLRET88	22.8%	40%	151 bp	9%	17.4
H2MF5DSXF_l03_n01_QG4702_POOLRET88	21.9%	41%	151 bp	18%	12.9
H2MF5DSXF_l03_n01_QG4706_POOLRET88	19.2%	39%	151 bp	9%	3.0
H2MF5DSXF_l03_n01_QG4713_POOLRET88	27.7%	40%	151 bp	9%	22.2
H2MF5DSXF_l03_n01_QG4714_POOLRET88	23.8%	40%	151 bp	9%	16.3
H2MF5DSXF_l03_n01_QG4715_POOLRET88	22.9%	40%	151 bp	9%	17.8
H2MF5DSXF_l03_n01_QG4718_POOLRET88	21.6%	41%	151 bp	9%	12.8
H2MF5DSXF_l03_n01_QG4730_POOLRET88	23.7%	40%	151 bp	9%	16.0
H2MF5DSXF_l03_n01_QG4733_POOLRET88	22.7%	40%	151 bp	9%	16.3
H2MF5DSXF_l03_n01_QG4739_POOLRET88	25.9%	40%	151 bp	18%	19.4
H2MF5DSXF_l03_n01_QG4740_POOLRET88	28.2%	40%	151 bp	18%	22.4
H2MF5DSXF_l03_n01_QG4741_POOLRET88	22.0%	40%	151 bp	9%	11.4
H2MF5DSXF_l03_n01_QG4742_POOLRET88	23.5%	40%	151 bp	9%	18.2
H2MF5DSXF_l03_n01_QG4754_POOLRET88	24.6%	41%	151 bp	9%	14.7
H2MF5DSXF_l03_n01_QG4759_POOLRET88	23.8%	40%	151 bp	18%	10.8
H2MF5DSXF_l03_n01_QG4779_POOLRET88	23.3%	41%	151 bp	18%	13.2
H2MF5DSXF_l03_n01_QG4780_POOLRET88	21.0%	41%	151 bp	9%	11.8
H2MF5DSXF_l03_n01_QG4781_POOLRET88	32.8%	40%	151 bp	9%	49.1
H2MF5DSXF_l03_n01_QG4782_POOLRET88	25.0%	40%	151 bp	9%	15.9
H2MF5DSXF_l03_n01_QG4790_POOLRET88	24.0%	40%	151 bp	9%	15.8
H2MF5DSXF_l03_n01_QG4805_POOLRET88	25.0%	39%	151 bp	9%	16.8
H2MF5DSXF_l03_n01_QG4817_POOLRET88	25.0%	40%	151 bp	9%	16.1
H2MF5DSXF_l03_n01_QG4824_POOLRET88	25.6%	40%	151 bp	9%	19.0
H2MF5DSXF_l03_n01_QG4838_POOLRET88	23.7%	39%	151 bp	9%	14.5
H2MF5DSXF_l03_n01_QG4847_POOLRET88	24.7%	39%	151 bp	9%	16.2
H2MF5DSXF_l03_n01_QG4866_POOLRET88	23.6%	40%	151 bp	9%	13.5
H2MF5DSXF_l03_n01_QG4867_POOLRET88	25.5%	40%	151 bp	9%	19.0
H2MF5DSXF_l03_n01_QG4868_POOLRET88	24.9%	40%	151 bp	9%	17.8
H2MF5DSXF_l03_n01_QG4869_POOLRET88	24.7%	40%	151 bp	9%	18.8
H2MF5DSXF_l03_n01_QG4870_POOLRET88	23.8%	41%	151 bp	9%	14.2
H2MF5DSXF_l03_n01_QG4871_POOLRET88	23.8%	42%	151 bp	9%	16.2
H2MF5DSXF_l03_n01_QG4876_POOLRET88	22.2%	40%	151 bp	9%	13.0
H2MF5DSXF_l03_n01_QG4880_POOLRET88	23.5%	39%	151 bp	9%	12.9
H2MF5DSXF_l03_n01_QG4891_POOLRET88	22.2%	40%	151 bp	9%	16.3
H2MF5DSXF_l03_n01_QG4892_POOLRET88	22.2%	41%	151 bp	9%	15.3
H2MF5DSXF_l03_n01_QG4893_POOLRET88	24.7%	40%	151 bp	9%	16.9
H2MF5DSXF_l03_n01_QG4894_POOLRET88	23.1%	41%	151 bp	9%	14.7
H2MF5DSXF_l03_n01_QG4896_POOLRET88	24.6%	39%	151 bp	9%	17.2
H2MF5DSXF_l03_n01_QG4897_POOLRET88	23.8%	40%	151 bp	9%	18.0
H2MF5DSXF_l03_n01_QG4904_POOLRET88	23.9%	40%	151 bp	9%	16.1
H2MF5DSXF_l03_n01_QG4910_POOLRET88	21.7%	40%	151 bp	9%	12.7
H2MF5DSXF_l03_n01_QG4924_POOLRET88	24.1%	40%	151 bp	9%	16.8
H2MF5DSXF_l03_n01_QG4947_POOLRET88	23.7%	40%	151 bp	9%	18.2
H2MF5DSXF_l03_n01_QG4952_POOLRET88	23.1%	39%	151 bp	9%	16.1
H2MF5DSXF_l03_n01_QG4954_POOLRET88	22.6%	39%	151 bp	9%	13.8
H2MF5DSXF_l03_n01_QG4956_POOLRET88	21.6%	40%	151 bp	9%	13.0
H2MF5DSXF_l03_n01_QG4966_POOLRET88	22.6%	40%	151 bp	9%	18.2
H2MF5DSXF_l03_n01_QG4968_POOLRET88	23.3%	41%	151 bp	9%	16.3
H2MF5DSXF_l03_n01_QG4996_POOLRET88	23.7%	40%	151 bp	9%	16.2
H2MF5DSXF_l03_n01_QG5008_POOLRET88	22.3%	40%	151 bp	9%	17.6
H2MF5DSXF_l03_n01_QG5094_POOLRET88	25.2%	40%	151 bp	9%	19.4
H2MF5DSXF_l03_n01_QG5103_POOLRET88	26.8%	40%	151 bp	9%	23.2
H2MF5DSXF_l03_n01_QG5104_POOLRET88	23.7%	40%	151 bp	18%	14.4
H2MF5DSXF_l03_n01_QG5149_POOLRET88	23.6%	41%	151 bp	9%	18.7
H2MF5DSXF_l03_n01_QG5150_POOLRET88	23.7%	40%	151 bp	9%	16.9
H2MF5DSXF_l03_n01_QG5153_POOLRET88	25.0%	40%	151 bp	9%	17.6
H2MF5DSXF_l03_n01_QG5159_POOLRET88	22.0%	42%	151 bp	9%	13.4
H2MF5DSXF_l03_n01_QG5160_POOLRET88	23.6%	41%	151 bp	9%	17.5
H2MF5DSXF_l03_n01_QG5161_POOLRET88	25.6%	41%	151 bp	9%	18.2
H2MF5DSXF_l03_n01_QG5166_POOLRET88	24.3%	39%	151 bp	9%	19.0
H2MF5DSXF_l03_n01_QG5169_POOLRET88	22.2%	40%	151 bp	9%	16.9
H2MF5DSXF_l03_n01_QG5170_POOLRET88	22.4%	40%	151 bp	9%	16.1
H2MF5DSXF_l03_n01_QG5179_POOLRET88	23.3%	39%	151 bp	9%	17.4
H2MF5DSXF_l03_n01_QG5180_POOLRET88	24.4%	39%	151 bp	9%	18.4
H2MF5DSXF_l03_n01_QG5187_POOLRET88	22.9%	41%	151 bp	9%	16.5
H2MF5DSXF_l03_n01_QG5197_POOLRET88	24.9%	40%	151 bp	9%	19.1
H2MF5DSXF_l03_n01_QG5198_POOLRET88	24.3%	40%	151 bp	9%	20.3
H2MF5DSXF_l03_n01_QG5205_POOLRET88	23.4%	40%	151 bp	9%	16.0
H2MF5DSXF_l03_n01_QG5220_POOLRET88	24.1%	38%	151 bp	9%	17.7
H2MF5DSXF_l03_n01_QG5237_POOLRET88	24.2%	39%	151 bp	9%	16.4
H2MF5DSXF_l03_n01_QG5256_POOLRET88	28.7%	40%	151 bp	9%	22.5
H2MF5DSXF_l03_n01_QG5260_POOLRET88	24.6%	40%	151 bp	9%	16.9
H2MF5DSXF_l03_n01_QG5262_POOLRET88	28.0%	40%	151 bp	9%	18.5
H2MF5DSXF_l03_n01_QG5277_POOLRET88	22.8%	41%	151 bp	9%	15.5
H2MF5DSXF_l03_n01_QG5284_POOLRET88	21.6%	39%	151 bp	9%	14.6
H2MF5DSXF_l03_n01_QG5286_POOLRET88	24.7%	39%	151 bp	9%	17.6
H2MF5DSXF_l03_n01_QG5290_POOLRET88	24.6%	40%	151 bp	9%	16.8
H2MF5DSXF_l03_n01_QG5295_POOLRET88	24.9%	40%	151 bp	9%	19.8
H2MF5DSXF_l03_n01_QG5300_POOLRET88	25.8%	41%	151 bp	9%	20.5
H2MF5DSXF_l03_n01_QG5305_POOLRET88	23.5%	38%	151 bp	9%	14.2
H2MF5DSXF_l03_n01_QG5319_POOLRET88	26.3%	38%	151 bp	9%	18.8
H2MF5DSXF_l03_n01_QG5348_POOLRET88	0.4%	24%	151 bp	27%	0.0
H2MF5DSXF_l03_n01_QG5351_POOLRET88	22.5%	39%	151 bp	18%	15.9
H2MF5DSXF_l03_n01_QG5364_POOLRET88	24.4%	37%	151 bp	9%	16.9
H2MF5DSXF_l03_n01_QG5365_POOLRET88	23.4%	40%	151 bp	9%	14.7
H2MF5DSXF_l03_n01_QG5366_POOLRET88	25.6%	41%	151 bp	9%	18.1
H2MF5DSXF_l03_n01_QG5369_POOLRET88	22.5%	40%	151 bp	9%	15.9
H2MF5DSXF_l03_n01_QG5405_POOLRET88	23.6%	40%	151 bp	9%	15.3
H2MF5DSXF_l03_n01_QG5406_POOLRET88	23.7%	39%	151 bp	9%	16.0
H2MF5DSXF_l03_n01_QG5471_POOLRET88	26.6%	39%	151 bp	9%	19.4
H2MF5DSXF_l03_n01_QG5474_POOLRET88	22.4%	40%	151 bp	9%	14.4
H2MF5DSXF_l03_n01_QG5485_POOLRET88	25.3%	40%	151 bp	9%	19.9
H2MF5DSXF_l03_n01_QG5488_POOLRET88	23.2%	41%	151 bp	9%	17.7
H2MF5DSXF_l03_n01_QG5502_POOLRET88	24.5%	40%	151 bp	9%	17.4
H2MF5DSXF_l03_n01_QG5503_POOLRET88	22.7%	40%	151 bp	9%	15.8
H2MF5DSXF_l03_n01_QG5506_POOLRET88	23.5%	40%	151 bp	9%	15.2
H2MF5DSXF_l03_n01_QG5507_POOLRET88	23.1%	40%	151 bp	9%	15.8
H2MF5DSXF_l03_n01_QG5510_POOLRET88	23.4%	41%	151 bp	9%	19.5
H2MF5DSXF_l03_n01_QG5512_POOLRET88	22.8%	40%	151 bp	9%	16.6
H2MF5DSXF_l03_n01_QG5525_POOLRET88	24.5%	40%	151 bp	9%	16.4
H2MF5DSXF_l03_n01_QG5531_POOLRET88	23.8%	39%	151 bp	9%	17.1
H2MF5DSXF_l03_n01_QG5534_POOLRET88	21.9%	41%	151 bp	9%	15.1
H2MF5DSXF_l03_n01_QG5539_POOLRET88	23.2%	39%	151 bp	9%	15.9
H2MF5DSXF_l03_n01_QG5550_POOLRET88	24.1%	40%	151 bp	9%	11.9
H2MF5DSXF_l03_n01_QG5551_POOLRET88	25.6%	39%	151 bp	9%	18.0
H2MF5DSXF_l03_n01_QG5552_POOLRET88	26.0%	38%	151 bp	9%	18.7
H2MF5DSXF_l03_n01_QG5556_POOLRET88	24.1%	40%	151 bp	9%	18.6
H2MF5DSXF_l03_n01_QG5576_POOLRET88	21.7%	41%	151 bp	9%	13.9
H2MF5DSXF_l03_n01_QG5577_POOLRET88	23.7%	39%	151 bp	9%	14.4
H2MF5DSXF_l03_n01_SOW17_POOLRET88	22.3%	40%	151 bp	9%	16.6
H2MF5DSXF_l03_n01_SOW18_POOLRET88	22.8%	39%	151 bp	18%	16.0
H2MF5DSXF_l03_n01_SOW19_POOLRET88	24.7%	39%	151 bp	9%	19.5
H2MF5DSXF_l03_n01_SOW21_POOLRET88	19.6%	39%	151 bp	18%	0.5
H2MF5DSXF_l03_n01_undetermined	76.4%	43%	151 bp	27%	324.2
H2MF5DSXF_l03_n02_ECA211_POOLRET88	24.0%	39%	151 bp	18%	14.9
H2MF5DSXF_l03_n02_ECA3023_POOLRET88	22.2%	41%	151 bp	18%	17.0
H2MF5DSXF_l03_n02_ECA3089_POOLRET88	21.2%	41%	151 bp	9%	3.6
H2MF5DSXF_l03_n02_ECA3092_POOLRET88	21.2%	41%	151 bp	18%	13.2
H2MF5DSXF_l03_n02_ECA3094_POOLRET88	23.5%	41%	151 bp	9%	17.4
H2MF5DSXF_l03_n02_ECA3212_POOLRET88	21.7%	39%	151 bp	18%	14.8
H2MF5DSXF_l03_n02_ECA3222_POOLRET88	20.6%	38%	151 bp	18%	10.9
H2MF5DSXF_l03_n02_ECA3230_POOLRET88	22.3%	38%	151 bp	9%	13.8
H2MF5DSXF_l03_n02_ECA3240_POOLRET88	20.5%	38%	151 bp	9%	11.5
H2MF5DSXF_l03_n02_ECA3252_POOLRET88	21.8%	38%	151 bp	9%	13.7
H2MF5DSXF_l03_n02_ECA3256_POOLRET88	25.3%	38%	151 bp	9%	22.3
H2MF5DSXF_l03_n02_ECA3529_POOLRET88	22.4%	38%	151 bp	18%	15.7
H2MF5DSXF_l03_n02_ECA3530_POOLRET88	22.5%	39%	151 bp	9%	14.5
H2MF5DSXF_l03_n02_ECA3531_POOLRET88	20.8%	40%	151 bp	18%	14.7
H2MF5DSXF_l03_n02_ECA3532_POOLRET88	23.2%	39%	151 bp	18%	18.6
H2MF5DSXF_l03_n02_ECA3533_POOLRET88	22.0%	39%	151 bp	18%	14.6
H2MF5DSXF_l03_n02_ECA3534_POOLRET88	22.4%	38%	151 bp	18%	16.3
H2MF5DSXF_l03_n02_ECA3535_POOLRET88	22.9%	38%	151 bp	18%	17.4
H2MF5DSXF_l03_n02_ECA3536_POOLRET88	20.2%	38%	151 bp	9%	7.7
H2MF5DSXF_l03_n02_ECA3537_POOLRET88	21.2%	39%	151 bp	18%	14.9
H2MF5DSXF_l03_n02_ECA3538_POOLRET88	21.1%	38%	151 bp	18%	14.4
H2MF5DSXF_l03_n02_ECA3539_POOLRET88	21.0%	40%	151 bp	18%	9.6
H2MF5DSXF_l03_n02_ECA3540_POOLRET88	22.6%	40%	151 bp	18%	17.6
H2MF5DSXF_l03_n02_ECA3541_POOLRET88	19.3%	40%	151 bp	9%	14.3
H2MF5DSXF_l03_n02_ECA3543_POOLRET88	25.9%	38%	151 bp	18%	17.3
H2MF5DSXF_l03_n02_ECA3544_POOLRET88	22.0%	40%	151 bp	9%	12.8
H2MF5DSXF_l03_n02_ECA3545_POOLRET88	19.2%	41%	151 bp	18%	12.8
H2MF5DSXF_l03_n02_ECA3546_POOLRET88	20.5%	39%	151 bp	18%	13.4
H2MF5DSXF_l03_n02_ECA3548_POOLRET88	24.1%	38%	151 bp	18%	19.1
H2MF5DSXF_l03_n02_ECA3549_POOLRET88	22.0%	39%	151 bp	18%	15.6
H2MF5DSXF_l03_n02_ECA3550_POOLRET88	20.4%	38%	151 bp	18%	8.8
H2MF5DSXF_l03_n02_ECA3551_POOLRET88	22.2%	38%	151 bp	18%	17.1
H2MF5DSXF_l03_n02_ECA3552_POOLRET88	20.5%	40%	151 bp	9%	13.6
H2MF5DSXF_l03_n02_ECA3553_POOLRET88	23.8%	40%	151 bp	18%	20.4
H2MF5DSXF_l03_n02_ECA3554_POOLRET88	23.1%	40%	151 bp	18%	16.6
H2MF5DSXF_l03_n02_ECA3555_POOLRET88	21.5%	40%	151 bp	18%	14.2
H2MF5DSXF_l03_n02_ECA3556_POOLRET88	22.5%	38%	151 bp	9%	12.7
H2MF5DSXF_l03_n02_ECA3557_POOLRET88	21.3%	40%	151 bp	9%	16.2
H2MF5DSXF_l03_n02_ECA3558_POOLRET88	21.3%	38%	151 bp	9%	14.7
H2MF5DSXF_l03_n02_ECA3560_POOLRET88	21.0%	40%	151 bp	18%	14.2
H2MF5DSXF_l03_n02_ECA3561_POOLRET88	22.6%	39%	151 bp	18%	15.2
H2MF5DSXF_l03_n02_ECA3562_POOLRET88	21.8%	39%	151 bp	18%	14.8
H2MF5DSXF_l03_n02_ECA3563_POOLRET88	23.1%	40%	151 bp	9%	15.5
H2MF5DSXF_l03_n02_ECA3564_POOLRET88	21.8%	40%	151 bp	18%	14.5
H2MF5DSXF_l03_n02_ECA3567_POOLRET88	21.1%	40%	151 bp	9%	15.8
H2MF5DSXF_l03_n02_ECA3568_POOLRET88	22.4%	39%	151 bp	9%	15.2
H2MF5DSXF_l03_n02_ECA3569_POOLRET88	21.7%	40%	151 bp	18%	17.0
H2MF5DSXF_l03_n02_ECA3570_POOLRET88	22.9%	38%	151 bp	18%	14.9
H2MF5DSXF_l03_n02_ECA3608_POOLRET88	19.6%	39%	151 bp	18%	12.7
H2MF5DSXF_l03_n02_ECA3707_POOLRET88	23.6%	40%	151 bp	18%	17.5
H2MF5DSXF_l03_n02_ECA3708_POOLRET88	21.0%	40%	151 bp	9%	7.7
H2MF5DSXF_l03_n02_ECA3709_POOLRET88	21.1%	41%	151 bp	18%	15.0
H2MF5DSXF_l03_n02_ECA3710_POOLRET88	18.9%	41%	151 bp	18%	11.5
H2MF5DSXF_l03_n02_ECA3711_POOLRET88	21.4%	41%	151 bp	9%	15.8
H2MF5DSXF_l03_n02_ECA3712_POOLRET88	21.1%	40%	151 bp	18%	13.1
H2MF5DSXF_l03_n02_ECA3713_POOLRET88	20.8%	41%	151 bp	18%	12.7
H2MF5DSXF_l03_n02_ECA3889_POOLRET88	21.0%	41%	151 bp	18%	13.6
H2MF5DSXF_l03_n02_NIC757_POOLRET88	21.9%	41%	151 bp	18%	18.9
H2MF5DSXF_l03_n02_QG4629_POOLRET88	24.0%	40%	151 bp	18%	13.5
H2MF5DSXF_l03_n02_QG4630_POOLRET88	20.3%	40%	151 bp	9%	12.9
H2MF5DSXF_l03_n02_QG4637_POOLRET88	23.2%	40%	151 bp	18%	20.9
H2MF5DSXF_l03_n02_QG4641_POOLRET88	20.9%	40%	151 bp	18%	11.2
H2MF5DSXF_l03_n02_QG4646_POOLRET88	20.8%	41%	151 bp	18%	11.7
H2MF5DSXF_l03_n02_QG4647_POOLRET88	22.6%	41%	151 bp	9%	18.7
H2MF5DSXF_l03_n02_QG4648_POOLRET88	21.4%	40%	151 bp	9%	14.4
H2MF5DSXF_l03_n02_QG4653_POOLRET88	22.1%	40%	151 bp	18%	13.9
H2MF5DSXF_l03_n02_QG4659_POOLRET88	25.8%	40%	151 bp	18%	20.3
H2MF5DSXF_l03_n02_QG4661_POOLRET88	22.0%	40%	151 bp	18%	13.4
H2MF5DSXF_l03_n02_QG4669_POOLRET88	21.3%	40%	151 bp	18%	13.4
H2MF5DSXF_l03_n02_QG4671_POOLRET88	25.6%	40%	151 bp	18%	23.2
H2MF5DSXF_l03_n02_QG4672_POOLRET88	24.6%	40%	151 bp	18%	23.8
H2MF5DSXF_l03_n02_QG4681_POOLRET88	21.4%	40%	151 bp	18%	17.1
H2MF5DSXF_l03_n02_QG4682_POOLRET88	21.6%	40%	151 bp	18%	14.1
H2MF5DSXF_l03_n02_QG4683_POOLRET88	25.7%	41%	151 bp	18%	20.0
H2MF5DSXF_l03_n02_QG4694_POOLRET88	21.6%	40%	151 bp	9%	14.4
H2MF5DSXF_l03_n02_QG4695_POOLRET88	24.1%	40%	151 bp	18%	19.8
H2MF5DSXF_l03_n02_QG4696_POOLRET88	23.7%	40%	151 bp	18%	16.0
H2MF5DSXF_l03_n02_QG4698_POOLRET88	20.5%	40%	151 bp	18%	14.7
H2MF5DSXF_l03_n02_QG4700_POOLRET88	21.0%	40%	151 bp	18%	14.4
H2MF5DSXF_l03_n02_QG4701_POOLRET88	22.4%	40%	151 bp	18%	17.4
H2MF5DSXF_l03_n02_QG4702_POOLRET88	21.5%	40%	151 bp	18%	12.9
H2MF5DSXF_l03_n02_QG4706_POOLRET88	18.8%	39%	151 bp	9%	3.0
H2MF5DSXF_l03_n02_QG4713_POOLRET88	27.0%	40%	151 bp	18%	22.2
H2MF5DSXF_l03_n02_QG4714_POOLRET88	23.3%	40%	151 bp	9%	16.3
H2MF5DSXF_l03_n02_QG4715_POOLRET88	22.3%	40%	151 bp	18%	17.8
H2MF5DSXF_l03_n02_QG4718_POOLRET88	21.0%	40%	151 bp	9%	12.8
H2MF5DSXF_l03_n02_QG4730_POOLRET88	23.3%	40%	151 bp	18%	16.0
H2MF5DSXF_l03_n02_QG4733_POOLRET88	22.2%	40%	151 bp	18%	16.3
H2MF5DSXF_l03_n02_QG4739_POOLRET88	25.1%	40%	151 bp	18%	19.4
H2MF5DSXF_l03_n02_QG4740_POOLRET88	27.5%	40%	151 bp	18%	22.4
H2MF5DSXF_l03_n02_QG4741_POOLRET88	21.4%	40%	151 bp	9%	11.4
H2MF5DSXF_l03_n02_QG4742_POOLRET88	22.8%	40%	151 bp	18%	18.2
H2MF5DSXF_l03_n02_QG4754_POOLRET88	24.1%	40%	151 bp	18%	14.7
H2MF5DSXF_l03_n02_QG4759_POOLRET88	23.4%	40%	151 bp	18%	10.8
H2MF5DSXF_l03_n02_QG4779_POOLRET88	23.0%	41%	151 bp	18%	13.2
H2MF5DSXF_l03_n02_QG4780_POOLRET88	20.6%	41%	151 bp	18%	11.8
H2MF5DSXF_l03_n02_QG4781_POOLRET88	31.2%	40%	151 bp	18%	49.1
H2MF5DSXF_l03_n02_QG4782_POOLRET88	23.9%	40%	151 bp	9%	15.9
H2MF5DSXF_l03_n02_QG4790_POOLRET88	23.2%	40%	151 bp	9%	15.8
H2MF5DSXF_l03_n02_QG4805_POOLRET88	24.5%	39%	151 bp	9%	16.8
H2MF5DSXF_l03_n02_QG4817_POOLRET88	24.5%	40%	151 bp	9%	16.1
H2MF5DSXF_l03_n02_QG4824_POOLRET88	24.6%	40%	151 bp	9%	19.0
H2MF5DSXF_l03_n02_QG4838_POOLRET88	22.8%	39%	151 bp	9%	14.5
H2MF5DSXF_l03_n02_QG4847_POOLRET88	23.6%	39%	151 bp	9%	16.2
H2MF5DSXF_l03_n02_QG4866_POOLRET88	22.9%	40%	151 bp	9%	13.5
H2MF5DSXF_l03_n02_QG4867_POOLRET88	24.0%	40%	151 bp	18%	19.0
H2MF5DSXF_l03_n02_QG4868_POOLRET88	24.4%	40%	151 bp	18%	17.8
H2MF5DSXF_l03_n02_QG4869_POOLRET88	24.1%	40%	151 bp	9%	18.8
H2MF5DSXF_l03_n02_QG4870_POOLRET88	23.5%	40%	151 bp	18%	14.2
H2MF5DSXF_l03_n02_QG4871_POOLRET88	23.0%	41%	151 bp	18%	16.2
H2MF5DSXF_l03_n02_QG4876_POOLRET88	21.5%	40%	151 bp	9%	13.0
H2MF5DSXF_l03_n02_QG4880_POOLRET88	23.1%	39%	151 bp	9%	12.9
H2MF5DSXF_l03_n02_QG4891_POOLRET88	21.5%	40%	151 bp	9%	16.3
H2MF5DSXF_l03_n02_QG4892_POOLRET88	21.7%	41%	151 bp	18%	15.3
H2MF5DSXF_l03_n02_QG4893_POOLRET88	23.9%	40%	151 bp	18%	16.9
H2MF5DSXF_l03_n02_QG4894_POOLRET88	22.4%	41%	151 bp	9%	14.7
H2MF5DSXF_l03_n02_QG4896_POOLRET88	23.9%	39%	151 bp	9%	17.2
H2MF5DSXF_l03_n02_QG4897_POOLRET88	23.1%	40%	151 bp	18%	18.0
H2MF5DSXF_l03_n02_QG4904_POOLRET88	23.3%	40%	151 bp	18%	16.1
H2MF5DSXF_l03_n02_QG4910_POOLRET88	21.4%	40%	151 bp	18%	12.7
H2MF5DSXF_l03_n02_QG4924_POOLRET88	23.3%	40%	151 bp	18%	16.8
H2MF5DSXF_l03_n02_QG4947_POOLRET88	22.6%	40%	151 bp	9%	18.2
H2MF5DSXF_l03_n02_QG4952_POOLRET88	22.7%	39%	151 bp	18%	16.1
H2MF5DSXF_l03_n02_QG4954_POOLRET88	22.1%	39%	151 bp	9%	13.8
H2MF5DSXF_l03_n02_QG4956_POOLRET88	21.2%	40%	151 bp	18%	13.0
H2MF5DSXF_l03_n02_QG4966_POOLRET88	22.2%	40%	151 bp	18%	18.2
H2MF5DSXF_l03_n02_QG4968_POOLRET88	22.6%	40%	151 bp	9%	16.3
H2MF5DSXF_l03_n02_QG4996_POOLRET88	23.1%	39%	151 bp	9%	16.2
H2MF5DSXF_l03_n02_QG5008_POOLRET88	21.9%	40%	151 bp	18%	17.6
H2MF5DSXF_l03_n02_QG5094_POOLRET88	24.5%	40%	151 bp	18%	19.4
H2MF5DSXF_l03_n02_QG5103_POOLRET88	26.2%	40%	151 bp	9%	23.2
H2MF5DSXF_l03_n02_QG5104_POOLRET88	23.2%	40%	151 bp	18%	14.4
H2MF5DSXF_l03_n02_QG5149_POOLRET88	23.0%	40%	151 bp	9%	18.7
H2MF5DSXF_l03_n02_QG5150_POOLRET88	23.1%	39%	151 bp	9%	16.9
H2MF5DSXF_l03_n02_QG5153_POOLRET88	24.4%	40%	151 bp	18%	17.6
H2MF5DSXF_l03_n02_QG5159_POOLRET88	21.3%	40%	151 bp	18%	13.4
H2MF5DSXF_l03_n02_QG5160_POOLRET88	23.2%	41%	151 bp	18%	17.5
H2MF5DSXF_l03_n02_QG5161_POOLRET88	25.2%	41%	151 bp	9%	18.2
H2MF5DSXF_l03_n02_QG5166_POOLRET88	23.6%	39%	151 bp	9%	19.0
H2MF5DSXF_l03_n02_QG5169_POOLRET88	21.6%	40%	151 bp	18%	16.9
H2MF5DSXF_l03_n02_QG5170_POOLRET88	21.9%	40%	151 bp	9%	16.1
H2MF5DSXF_l03_n02_QG5179_POOLRET88	22.9%	40%	151 bp	18%	17.4
H2MF5DSXF_l03_n02_QG5180_POOLRET88	23.7%	39%	151 bp	9%	18.4
H2MF5DSXF_l03_n02_QG5187_POOLRET88	22.4%	41%	151 bp	18%	16.5
H2MF5DSXF_l03_n02_QG5197_POOLRET88	24.2%	40%	151 bp	18%	19.1
H2MF5DSXF_l03_n02_QG5198_POOLRET88	23.9%	39%	151 bp	9%	20.3
H2MF5DSXF_l03_n02_QG5205_POOLRET88	23.0%	40%	151 bp	9%	16.0
H2MF5DSXF_l03_n02_QG5220_POOLRET88	23.5%	38%	151 bp	18%	17.7
H2MF5DSXF_l03_n02_QG5237_POOLRET88	23.8%	39%	151 bp	18%	16.4
H2MF5DSXF_l03_n02_QG5256_POOLRET88	27.8%	40%	151 bp	9%	22.5
H2MF5DSXF_l03_n02_QG5260_POOLRET88	24.0%	39%	151 bp	9%	16.9
H2MF5DSXF_l03_n02_QG5262_POOLRET88	27.4%	40%	151 bp	18%	18.5
H2MF5DSXF_l03_n02_QG5277_POOLRET88	22.2%	41%	151 bp	18%	15.5
H2MF5DSXF_l03_n02_QG5284_POOLRET88	20.7%	40%	151 bp	9%	14.6
H2MF5DSXF_l03_n02_QG5286_POOLRET88	23.9%	39%	151 bp	9%	17.6
H2MF5DSXF_l03_n02_QG5290_POOLRET88	23.6%	40%	151 bp	9%	16.8
H2MF5DSXF_l03_n02_QG5295_POOLRET88	24.2%	40%	151 bp	9%	19.8
H2MF5DSXF_l03_n02_QG5300_POOLRET88	25.1%	40%	151 bp	18%	20.5
H2MF5DSXF_l03_n02_QG5305_POOLRET88	23.1%	38%	151 bp	9%	14.2
H2MF5DSXF_l03_n02_QG5319_POOLRET88	25.6%	38%	151 bp	9%	18.8
H2MF5DSXF_l03_n02_QG5348_POOLRET88	0.4%	25%	151 bp	18%	0.0
H2MF5DSXF_l03_n02_QG5351_POOLRET88	22.1%	39%	151 bp	18%	15.9
H2MF5DSXF_l03_n02_QG5364_POOLRET88	23.8%	37%	151 bp	9%	16.9
H2MF5DSXF_l03_n02_QG5365_POOLRET88	23.0%	40%	151 bp	9%	14.7
H2MF5DSXF_l03_n02_QG5366_POOLRET88	24.9%	40%	151 bp	18%	18.1
H2MF5DSXF_l03_n02_QG5369_POOLRET88	22.1%	39%	151 bp	9%	15.9
H2MF5DSXF_l03_n02_QG5405_POOLRET88	23.0%	40%	151 bp	9%	15.3
H2MF5DSXF_l03_n02_QG5406_POOLRET88	22.8%	39%	151 bp	9%	16.0
H2MF5DSXF_l03_n02_QG5471_POOLRET88	25.7%	39%	151 bp	9%	19.4
H2MF5DSXF_l03_n02_QG5474_POOLRET88	22.1%	40%	151 bp	18%	14.4
H2MF5DSXF_l03_n02_QG5485_POOLRET88	24.6%	40%	151 bp	9%	19.9
H2MF5DSXF_l03_n02_QG5488_POOLRET88	22.6%	41%	151 bp	18%	17.7
H2MF5DSXF_l03_n02_QG5502_POOLRET88	23.2%	40%	151 bp	9%	17.4
H2MF5DSXF_l03_n02_QG5503_POOLRET88	22.1%	40%	151 bp	9%	15.8
H2MF5DSXF_l03_n02_QG5506_POOLRET88	22.5%	40%	151 bp	9%	15.2
H2MF5DSXF_l03_n02_QG5507_POOLRET88	22.8%	40%	151 bp	9%	15.8
H2MF5DSXF_l03_n02_QG5510_POOLRET88	23.2%	40%	151 bp	9%	19.5
H2MF5DSXF_l03_n02_QG5512_POOLRET88	22.4%	40%	151 bp	18%	16.6
H2MF5DSXF_l03_n02_QG5525_POOLRET88	23.9%	40%	151 bp	18%	16.4
H2MF5DSXF_l03_n02_QG5531_POOLRET88	23.2%	39%	151 bp	9%	17.1
H2MF5DSXF_l03_n02_QG5534_POOLRET88	21.5%	40%	151 bp	18%	15.1
H2MF5DSXF_l03_n02_QG5539_POOLRET88	22.6%	39%	151 bp	9%	15.9
H2MF5DSXF_l03_n02_QG5550_POOLRET88	23.3%	40%	151 bp	9%	11.9
H2MF5DSXF_l03_n02_QG5551_POOLRET88	24.9%	39%	151 bp	9%	18.0
H2MF5DSXF_l03_n02_QG5552_POOLRET88	25.3%	39%	151 bp	9%	18.7
H2MF5DSXF_l03_n02_QG5556_POOLRET88	23.5%	40%	151 bp	9%	18.6
H2MF5DSXF_l03_n02_QG5576_POOLRET88	21.3%	41%	151 bp	18%	13.9
H2MF5DSXF_l03_n02_QG5577_POOLRET88	23.2%	39%	151 bp	9%	14.4
H2MF5DSXF_l03_n02_SOW17_POOLRET88	22.0%	40%	151 bp	18%	16.6
H2MF5DSXF_l03_n02_SOW18_POOLRET88	22.4%	39%	151 bp	9%	16.0
H2MF5DSXF_l03_n02_SOW19_POOLRET88	23.7%	39%	151 bp	18%	19.5
H2MF5DSXF_l03_n02_SOW21_POOLRET88	19.4%	39%	151 bp	18%	0.5
H2MF5DSXF_l03_n02_undetermined	73.5%	43%	151 bp	27%	324.2

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Sort	Group	Column	Description	ID	Scale
\|\|	FastQC	% Dups	% Duplicate Reads	`percent_duplicates`	None
\|\|	FastQC	% GC	Average % GC Content	`percent_gc`	None
\|\|	FastQC	Length	Average Sequence Length (bp)	`avg_sequence_length`	None
\|\|	FastQC	% Failed	Percentage of modules failed in FastQC report (includes those not plotted here)	`percent_fails`	None
\|\|	FastQC	M Seqs	Total Sequences (millions)	`total_sequences`	read_count

Demultiplexing Report

Total Read Count: Total number of PF (Passing Filter) reads in this library.
Portion: The proportion of reads that represent the individual library in the entire Library Pool.

Showing ¹⁹³/₁₉₃ rows and ²/₂ columns.

Library	Total Read Count	Portion (%)
ECA211_POOLRET88	14933839	0.4
ECA3023_POOLRET88	17042072	0.5
ECA3089_POOLRET88	3604493	0.1
ECA3092_POOLRET88	13215770	0.4
ECA3094_POOLRET88	17356317	0.5
ECA3212_POOLRET88	14834441	0.4
ECA3222_POOLRET88	10907401	0.3
ECA3230_POOLRET88	13770237	0.4
ECA3240_POOLRET88	11544736	0.3
ECA3252_POOLRET88	13683223	0.4
ECA3256_POOLRET88	22315406	0.7
ECA3529_POOLRET88	15746047	0.5
ECA3530_POOLRET88	14541800	0.4
ECA3531_POOLRET88	14680292	0.4
ECA3532_POOLRET88	18621621	0.6
ECA3533_POOLRET88	14605869	0.4
ECA3534_POOLRET88	16333827	0.5
ECA3535_POOLRET88	17354955	0.5
ECA3536_POOLRET88	7689185	0.2
ECA3537_POOLRET88	14923581	0.4
ECA3538_POOLRET88	14384496	0.4
ECA3539_POOLRET88	9641512	0.3
ECA3540_POOLRET88	17635067	0.5
ECA3541_POOLRET88	14291895	0.4
ECA3543_POOLRET88	17334990	0.5
ECA3544_POOLRET88	12832916	0.4
ECA3545_POOLRET88	12811865	0.4
ECA3546_POOLRET88	13443445	0.4
ECA3548_POOLRET88	19073356	0.6
ECA3549_POOLRET88	15637108	0.5
ECA3550_POOLRET88	8836492	0.3
ECA3551_POOLRET88	17096113	0.5
ECA3552_POOLRET88	13577750	0.4
ECA3553_POOLRET88	20378586	0.6
ECA3554_POOLRET88	16567012	0.5
ECA3555_POOLRET88	14226485	0.4
ECA3556_POOLRET88	12688276	0.4
ECA3557_POOLRET88	16212283	0.5
ECA3558_POOLRET88	14730995	0.4
ECA3560_POOLRET88	14156427	0.4
ECA3561_POOLRET88	15159237	0.4
ECA3562_POOLRET88	14809588	0.4
ECA3563_POOLRET88	15495339	0.5
ECA3564_POOLRET88	14498411	0.4
ECA3567_POOLRET88	15768353	0.5
ECA3568_POOLRET88	15193447	0.5
ECA3569_POOLRET88	17009180	0.5
ECA3570_POOLRET88	14901483	0.4
ECA3608_POOLRET88	12670008	0.4
ECA3707_POOLRET88	17523446	0.5
ECA3708_POOLRET88	7730443	0.2
ECA3709_POOLRET88	14959341	0.4
ECA3710_POOLRET88	11487535	0.3
ECA3711_POOLRET88	15783886	0.5
ECA3712_POOLRET88	13127408	0.4
ECA3713_POOLRET88	12729781	0.4
ECA3889_POOLRET88	13627898	0.4
NIC757_POOLRET88	18868089	0.6
QG4629_POOLRET88	13515641	0.4
QG4630_POOLRET88	12872531	0.4
QG4637_POOLRET88	20850799	0.6
QG4641_POOLRET88	11176238	0.3
QG4646_POOLRET88	11690137	0.3
QG4647_POOLRET88	18658998	0.6
QG4648_POOLRET88	14391295	0.4
QG4653_POOLRET88	13919268	0.4
QG4659_POOLRET88	20340742	0.6
QG4661_POOLRET88	13424099	0.4
QG4669_POOLRET88	13441650	0.4
QG4671_POOLRET88	23189664	0.7
QG4672_POOLRET88	23750196	0.7
QG4681_POOLRET88	17108873	0.5
QG4682_POOLRET88	14086952	0.4
QG4683_POOLRET88	20005179	0.6
QG4694_POOLRET88	14355371	0.4
QG4695_POOLRET88	19799884	0.6
QG4696_POOLRET88	16009013	0.5
QG4698_POOLRET88	14712865	0.4
QG4700_POOLRET88	14386337	0.4
QG4701_POOLRET88	17358853	0.5
QG4702_POOLRET88	12880366	0.4
QG4706_POOLRET88	3008106	0.1
QG4713_POOLRET88	22171419	0.7
QG4714_POOLRET88	16272229	0.5
QG4715_POOLRET88	17833559	0.5
QG4718_POOLRET88	12764633	0.4
QG4730_POOLRET88	15991322	0.5
QG4733_POOLRET88	16256731	0.5
QG4739_POOLRET88	19361917	0.6
QG4740_POOLRET88	22355246	0.7
QG4741_POOLRET88	11397696	0.3
QG4742_POOLRET88	18214199	0.5
QG4754_POOLRET88	14720117	0.4
QG4759_POOLRET88	10797338	0.3
QG4779_POOLRET88	13184541	0.4
QG4780_POOLRET88	11805059	0.3
QG4781_POOLRET88	49103870	1.5
QG4782_POOLRET88	15949917	0.5
QG4790_POOLRET88	15783065	0.5
QG4805_POOLRET88	16785458	0.5
QG4817_POOLRET88	16067437	0.5
QG4824_POOLRET88	19008311	0.6
QG4838_POOLRET88	14489223	0.4
QG4847_POOLRET88	16200317	0.5
QG4866_POOLRET88	13465870	0.4
QG4867_POOLRET88	19010141	0.6
QG4868_POOLRET88	17750566	0.5
QG4869_POOLRET88	18804449	0.6
QG4870_POOLRET88	14155325	0.4
QG4871_POOLRET88	16223620	0.5
QG4876_POOLRET88	13047096	0.4
QG4880_POOLRET88	12899897	0.4
QG4891_POOLRET88	16318434	0.5
QG4892_POOLRET88	15252549	0.5
QG4893_POOLRET88	16915508	0.5
QG4894_POOLRET88	14724199	0.4
QG4896_POOLRET88	17212538	0.5
QG4897_POOLRET88	17979282	0.5
QG4904_POOLRET88	16143588	0.5
QG4910_POOLRET88	12720748	0.4
QG4924_POOLRET88	16778502	0.5
QG4947_POOLRET88	18231754	0.5
QG4952_POOLRET88	16102876	0.5
QG4954_POOLRET88	13800694	0.4
QG4956_POOLRET88	13011507	0.4
QG4966_POOLRET88	18187490	0.5
QG4968_POOLRET88	16346643	0.5
QG4996_POOLRET88	16213999	0.5
QG5008_POOLRET88	17632560	0.5
QG5094_POOLRET88	19352448	0.6
QG5103_POOLRET88	23192172	0.7
QG5104_POOLRET88	14357850	0.4
QG5149_POOLRET88	18653816	0.6
QG5150_POOLRET88	16892330	0.5
QG5153_POOLRET88	17620934	0.5
QG5159_POOLRET88	13428804	0.4
QG5160_POOLRET88	17470244	0.5
QG5161_POOLRET88	18173181	0.5
QG5166_POOLRET88	18974384	0.6
QG5169_POOLRET88	16914976	0.5
QG5170_POOLRET88	16069472	0.5
QG5179_POOLRET88	17395980	0.5
QG5180_POOLRET88	18424520	0.5
QG5187_POOLRET88	16542580	0.5
QG5197_POOLRET88	19098716	0.6
QG5198_POOLRET88	20251299	0.6
QG5205_POOLRET88	16049200	0.5
QG5220_POOLRET88	17747071	0.5
QG5237_POOLRET88	16380975	0.5
QG5256_POOLRET88	22452376	0.7
QG5260_POOLRET88	16945423	0.5
QG5262_POOLRET88	18496215	0.5
QG5277_POOLRET88	15513915	0.5
QG5284_POOLRET88	14579301	0.4
QG5286_POOLRET88	17631836	0.5
QG5290_POOLRET88	16769681	0.5
QG5295_POOLRET88	19758184	0.6
QG5300_POOLRET88	20521352	0.6
QG5305_POOLRET88	14222032	0.4
QG5319_POOLRET88	18778480	0.6
QG5348_POOLRET88	1851	0.0
QG5351_POOLRET88	15920361	0.5
QG5364_POOLRET88	16855687	0.5
QG5365_POOLRET88	14667775	0.4
QG5366_POOLRET88	18056243	0.5
QG5369_POOLRET88	15864240	0.5
QG5405_POOLRET88	15304330	0.5
QG5406_POOLRET88	15951966	0.5
QG5471_POOLRET88	19382757	0.6
QG5474_POOLRET88	14400801	0.4
QG5485_POOLRET88	19896428	0.6
QG5488_POOLRET88	17689422	0.5
QG5502_POOLRET88	17413722	0.5
QG5503_POOLRET88	15779017	0.5
QG5506_POOLRET88	15199564	0.5
QG5507_POOLRET88	15803727	0.5
QG5510_POOLRET88	19538692	0.6
QG5512_POOLRET88	16581487	0.5
QG5525_POOLRET88	16358795	0.5
QG5531_POOLRET88	17118761	0.5
QG5534_POOLRET88	15104786	0.4
QG5539_POOLRET88	15875120	0.5
QG5550_POOLRET88	11874038	0.4
QG5551_POOLRET88	17993819	0.5
QG5552_POOLRET88	18724205	0.6
QG5556_POOLRET88	18610236	0.6
QG5576_POOLRET88	13940006	0.4
QG5577_POOLRET88	14384877	0.4
SOW17_POOLRET88	16558731	0.5
SOW18_POOLRET88	15983298	0.5
SOW19_POOLRET88	19532529	0.6
SOW21_POOLRET88	487689	0.0
undetermined	324217635	9.6

Uncheck the tick box to hide columns. Click and drag the handle on the left to change order.

Sort	Visible	Group	Column	Description	ID	Scale
\|\|			Total Read Count	Total Read Count	`Total Read Count`	None
\|\|			Portion (%)	Portion (%)	`Portion (%)`	None

Barcodes of Undetermined Reads

We have determined the barcodes of your undetermined reads. Here are the top 20 barcodes. The full list is available here.

Showing ²⁰/₂₀ rows and ²/₂ columns.

Barcode Sequence(s)	Count	Frequency (%)
GGGGGGGG-AGATCTCG	233179690.0	71.9
TCGTCTGA-GGGGGGGG	711160.0	0.2
GGGGGGGG-CGATCTCG	606572.0	0.2
CAGTTCAA-GGCGTTAT	456212.0	0.1
TCTGGAAC-AGTGCTAG	433224.0	0.1
CGAGAGAA-GGGGGGGG	387148.0	0.1
GAAGCCTA-ATACTGAC	379334.0	0.1
GGGGGGGG-NGATCTCG	347220.0	0.1
TCACGCCA-AGGAACAT	317767.0	0.1
GGGGGGGG-GGGGGGGG	252301.0	0.1
CCAAGGTT-GGGGGGGG	239177.0	0.1
GGGGGGGG-GACTGCTG	205095.0	0.1
GTACCACA-GTTGTGGG	205034.0	0.1
TCTGGAAC-AGTCTAGT	173072.0	0.1
GGGGGGGG-TCAAGGAC	164172.0	0.1
GGGGGGGG-GTTAAGGC	161874.0	0.1
GGGGGGGG-ACGGTCTT	159851.0	0.1
GACACAAA-TCGTCAAG	159521.0	0.1
GGGGGGGG-AGGTCACT	156925.0	0.1
GGGGGGGG-AAGTCGAG	154838.0	0.1

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Sort	Visible	Group	Column	Description	ID	Scale
\|\|			Count	Count	`Count`	None
\|\|			Frequency (%)	Frequency (%)	`Frequency (%)`	None

Run Statistics

Showing ¹/₁ rows and ⁴/₄ columns.

Lane	Total # of Single-End Reads	Total # PF Reads	% Undetermined	% PhiX Aligned
3.0	3830022144	3374713892	9.6	6.9

Uncheck the tick box to hide columns. Click and drag the handle on the left to change order.

Sort	Column	Description	ID	Scale
\|\|	Total # of Single-End Reads	Total # of Single-End Reads	`Total # of Single-End Reads`	None
\|\|	Total # PF Reads	Total # PF Reads	`Total # PF Reads`	None
\|\|	% Undetermined	% Undetermined	`% Undetermined`	None
\|\|	% PhiX Aligned	% PhiX Aligned	`% PhiX Aligned`	None

FastQC

FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

Sequence Counts

Sequence counts for each sample. Duplicate read counts are an estimate only.

This plot show the total number of reads, broken down into unique and duplicate if possible (only more recent versions of FastQC give duplicate info).

You can read more about duplicate calculation in the FastQC documentation. A small part has been copied here for convenience:

Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

Sequence Quality Histograms

The mean quality value across each base position in the read.

To enable multiple samples to be plotted on the same graph, only the mean quality scores are plotted (unlike the box plots seen in FastQC reports).

Taken from the FastQC help:

The y-axis on the graph shows the quality scores. The higher the score, the better the base call. The background of the graph divides the y axis into very good quality calls (green), calls of reasonable quality (orange), and calls of poor quality (red). The quality of calls on most platforms will degrade as the run progresses, so it is common to see base calls falling into the orange area towards the end of a read.

Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

Per Sequence Quality Scores

The number of reads with average quality scores. Shows if a subset of reads has poor quality.

From the FastQC help:

The per sequence quality score report allows you to see if a subset of your sequences have universally low quality values. It is often the case that a subset of sequences will have universally poor quality, however these should represent only a small percentage of the total sequences.

Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

Per Base Sequence Content

The proportion of each base position for which each of the four normal DNA bases has been called.

To enable multiple samples to be shown in a single plot, the base composition data is shown as a heatmap. The colours represent the balance between the four bases: an even distribution should give an even muddy brown colour. Hover over the plot to see the percentage of the four bases under the cursor.

To see the data as a line plot, as in the original FastQC graph, click on a sample track.

From the FastQC help:

Per Base Sequence Content plots out the proportion of each base position in a file for which each of the four normal DNA bases has been called.

In a random library you would expect that there would be little to no difference between the different bases of a sequence run, so the lines in this plot should run parallel with each other. The relative amount of each base should reflect the overall amount of these bases in your genome, but in any case they should not be hugely imbalanced from each other.

It's worth noting that some types of library will always produce biased sequence composition, normally at the start of the read. Libraries produced by priming using random hexamers (including nearly all RNA-Seq libraries) and those which were fragmented using transposases inherit an intrinsic bias in the positions at which reads start. This bias does not concern an absolute sequence, but instead provides enrichement of a number of different K-mers at the 5' end of the reads. Whilst this is a true technical bias, it isn't something which can be corrected by trimming and in most cases doesn't seem to adversely affect the downstream analysis.

Click a sample row to see a line plot for that dataset.

Rollover for sample name

Position: -

%T: -

%C: -

%A: -

%G: -

Per Sequence GC Content

The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content.

From the FastQC help:

This module measures the GC content across the whole length of each sequence in a file and compares it to a modelled normal distribution of GC content.

In a normal random library you would expect to see a roughly normal distribution of GC content where the central peak corresponds to the overall GC content of the underlying genome. Since we don't know the the GC content of the genome the modal GC content is calculated from the observed data and used to build a reference distribution.

An unusually shaped distribution could indicate a contaminated library or some other kinds of biased subset. A normal distribution which is shifted indicates some systematic bias which is independent of base position. If there is a systematic bias which creates a shifted normal distribution then this won't be flagged as an error by the module since it doesn't know what your genome's GC content should be.

Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

Per Base N Content

The percentage of base calls at each position for which an N was called.

From the FastQC help:

If a sequencer is unable to make a base call with sufficient confidence then it will normally substitute an N rather than a conventional base call. This graph shows the percentage of base calls at each position for which an N was called.

It's not unusual to see a very low proportion of Ns appearing in a sequence, especially nearer the end of a sequence. However, if this proportion rises above a few percent it suggests that the analysis pipeline was unable to interpret the data well enough to make valid base calls.

Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

Sequence Length Distribution

All samples have sequences of a single length (151bp).

Sequence Duplication Levels

The relative level of duplication found for every sequence.

From the FastQC Help:

In a diverse library most sequences will occur only once in the final set. A low level of duplication may indicate a very high level of coverage of the target sequence, but a high level of duplication is more likely to indicate some kind of enrichment bias (eg PCR over amplification). This graph shows the degree of duplication for every sequence in a library: the relative number of sequences with different degrees of duplication.

The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

In a properly diverse library most sequences should fall into the far left of the plot in both the red and blue lines. A general level of enrichment, indicating broad oversequencing in the library will tend to flatten the lines, lowering the low end and generally raising other categories. More specific enrichments of subsets, or the presence of low complexity contaminants will tend to produce spikes towards the right of the plot.

Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

Overrepresented sequences

The total amount of overrepresented sequences found in each library.

FastQC calculates and lists overrepresented sequences in FastQ files. It would not be possible to show this for all samples in a MultiQC report, so instead this plot shows the number of sequences categorized as over represented.

Sometimes, a single sequence may account for a large number of reads in a dataset. To show this, the bars are split into two: the first shows the overrepresented reads that come from the single most common sequence. The second shows the total count from all remaining overrepresented sequences.

From the FastQC Help:

A normal high-throughput library will contain a diverse set of sequences, with no individual sequence making up a tiny fraction of the whole. Finding that a single sequence is very overrepresented in the set either means that it is highly biologically significant, or indicates that the library is contaminated, or not as diverse as you expected.

FastQC lists all of the sequences which make up more than 0.1% of the total. To conserve memory only sequences which appear in the first 100,000 sequences are tracked to the end of the file. It is therefore possible that a sequence which is overrepresented but doesn't appear at the start of the file for some reason could be missed by this module.

386 samples had less than 1% of reads made up of overrepresented sequences

Adapter Content

The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position.

Note that only samples with ≥ 0.1% adapter contamination are shown.

There may be several lines per sample, as one is shown for each adapter detected in the file.

From the FastQC Help:

The plot shows a cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. Once a sequence has been seen in a read it is counted as being present right through to the end of the read so the percentages you see will only increase as the read length goes on.

Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).

Status Checks

Status for each FastQC section showing whether results seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

FastQC assigns a status for each section of the report. These give a quick evaluation of whether the results of the analysis seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

It is important to stress that although the analysis results appear to give a pass/fail result, these evaluations must be taken in the context of what you expect from your library. A 'normal' sample as far as FastQC is concerned is random and diverse. Some experiments may be expected to produce libraries which are biased in particular ways. You should treat the summary evaluations therefore as pointers to where you should concentrate your attention and understand why your library may not look random and diverse.

Specific guidance on how to interpret the output of each module can be found in the relevant report section, or in the FastQC help.

In this heatmap, we summarise all of these into a single heatmap for a quick overview. Note that not all FastQC sections have plots in MultiQC reports, but all status checks are shown in this heatmap.

v1.9

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Per Base Sequence Content

Rollover for sample name

Per Sequence GC Content

Per Base N Content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

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Status Checks

Toggle navigation v1.9

MultiQC Toolbox

Apply Highlight Samples

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About MultiQC

General Statistics

General Statistics: Columns

Demultiplexing Report

Table Hzuf: Columns

Barcodes of Undetermined Reads

Table Xygh: Columns

Run Statistics

Table Tixa: Columns

FastQC

Sequence Counts Help

Sequence Quality Histograms Help

Per Sequence Quality Scores Help

Per Base Sequence Content Help

Rollover for sample name

Per Sequence GC Content Help

Per Base N Content Help

Sequence Length Distribution

Sequence Duplication Levels Help

Overrepresented sequences Help

Adapter Content Help

Status Checks Help

v1.9

Highlight Samples

Rename Samples

Show / Hide Samples

Sequence Counts

Sequence Quality Histograms

Per Sequence Quality Scores

Per Base Sequence Content

Per Sequence GC Content

Per Base N Content

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Status Checks