FastQCFastQC Report
Thu 30 Jan 2025
H2MF5DSXF_l03_n01_QG5348_POOLRET88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2MF5DSXF_l03_n01_QG5348_POOLRET88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1851
Sequences flagged as poor quality0
Sequence length151
%GC24

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCGAAACGTCGCGGAGCCGGAAATTCTGAAGATCTGAAATTCAGGAA40.21609940572663425No Hit
CCAAATACTGTCATCGGCAAATGCTAAAATTTACTAGAACCGAAAATTTT30.1620745542949757No Hit
ATAAACAGTGAGAACCTAATATGGGAATTTTGGGATTTTTTTTTGGAATA20.10804970286331712No Hit
CCTGTATCTTCAAAGATATCTACTTGGAAACTCCAGAATTTCTGAAATAC20.10804970286331712No Hit
GAGAAGATCCCCAAAAATGTAACTGTAAAACAAAAATATTGAGTTATTAT20.10804970286331712No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)