FastQCFastQC Report
Fri 9 Feb 2018
H2LMFBGX5_n02_atac_whole_root_n7_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2LMFBGX5_n02_atac_whole_root_n7_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90349030
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1803720.19963911067999293No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG161050.019.7263135
CTCGGTG724300.017.82309569
TCTCGGT992350.017.21638569
AGTGTAG4577800.016.84822369
GTGTAGA3513800.016.46713869
TCGGTGG422700.015.804728569
TGTAGAT2727800.015.02984169
GAGTGTA5759750.014.88441669
CTAGTAC194300.014.4510723
TACCCCG128800.014.0869485
TACACCG150000.013.8896945
TAGTACT234600.013.5566254
ATACCGT134900.013.475546
ATACCGC180400.013.3273486
GTAGATC2151500.013.25608969
ATCTCGG1309150.013.15981468
TAGACCG176500.012.9377965
AGTACTA221750.012.9266045
GGATAGA273950.012.6813241
CGAGTGT7077700.012.61348269
Produced by FastQC (version 0.11.2)