FastQCFastQC Report
Fri 9 Feb 2018
H2LMFBGX5_n02_atac_whole_root_n7_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2LMFBGX5_n02_atac_whole_root_n7_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85756217
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1699970.1982328581495147No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG209550.022.1895055
AGTGTAG2854350.021.05765769
GTGTAGA1993300.020.84014169
GAGTGTA3849050.018.89798269
ATACCGC175550.017.880776
GCACGCC268800.016.80189130
ATACCGT149150.016.0733816
TGTAGAT1459450.015.91509969
TAGGCGG309000.015.695454
CGAGTGT5068550.015.45267769
CTCGGTG363300.015.43501169
TACCCCG132250.014.9452915
TCGCGCG84550.014.4807579
TAGTACT333050.014.3652764
TACCGCC200600.014.2554337
CTAGTAC279700.014.1948753
AGACCGT200350.014.186776
GTCGCGC77100.014.1807238
GCGGATC331300.013.9417087
TAATACT169250.013.879234
Produced by FastQC (version 0.11.2)