FastQCFastQC Report
Fri 9 Feb 2018
H2LMFBGX5_n02_atac_whole_root_col-0_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2LMFBGX5_n02_atac_whole_root_col-0_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68923062
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG164500.021.4094075
AGTGTAG2014450.020.09196369
GTGTAGA1447900.019.85291769
GAGTGTA2701050.018.46346569
GCACGCC215050.016.81397430
TAGGCGG238500.016.2997514
ATACCGC142950.016.2396376
TCGCGCG68600.015.6857179
CGAGTGT3563500.015.6798469
TGTAGAT1084750.015.59096269
GTCGCGC65600.015.5099718
CTAGTAC220750.014.8602853
ATACCGT125750.014.7028736
GCGGATC252450.014.47027
TACCCCG96800.014.3606635
TAGACCG145950.014.1332445
TAGTACT285900.013.814554
AGACCGT163250.013.75546
GTAGATC797500.013.73823469
ACGAGTG4274900.013.59116469
Produced by FastQC (version 0.11.2)