FastQCFastQC Report
Fri 9 Feb 2018
H2LMFBGX5_n02_atac_whole_root_col-0_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2LMFBGX5_n02_atac_whole_root_col-0_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94183528
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2206110.23423522635507987No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG251500.027.0194455
CTCGGTG577600.024.97285769
TAGGCGG470050.022.360354
ATACCGC235000.022.1204666
GCACGCC467850.021.54115930
GCGGATC501200.020.3722697
GTGTAGA4268950.019.28636269
TCGCGCG94250.018.4062029
AGTGTAG5812350.018.2711369
GGCGGAT587350.018.123746
GCCCATC501000.018.10557234
AGGCCGC133250.018.0950096
ACGAAGC575900.017.98718325
TAGGCCG182800.017.5679745
CGAAGCA590800.017.52197826
GAAGCAC592450.017.47890927
TACCGCC282600.017.3208457
AGGCGGA604150.017.24295
TTTATAC180550.017.1182773
ACGCCCA528100.017.03932632
Produced by FastQC (version 0.11.2)