FastQCFastQC Report
Fri 9 Feb 2018
H2LMFBGX5_n01_atac_whole_root_n7_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2LMFBGX5_n01_atac_whole_root_n7_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90349030
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG154850.018.6684685
CTCCTGA3596100.016.01052969
GCATCTC852400.015.90963869
TCCTGAG2690250.015.0817669
ACTCCTG5239250.014.95750869
GACTCCT5953400.014.56188769
TAGTACT238200.014.438784
CTAGTAC195650.014.3348543
TACCCCG122600.014.125025
TACACCG148850.013.8125315
TAGACCG178700.013.7831845
CCTGAGC2137750.013.58662669
CATCTCG781650.013.5751369
AGTACTA216750.013.3211665
GAGCATC1092950.013.28045467
AGACCGT227300.013.264546
GGATAGA279350.013.2339381
GTATAGA170800.013.0959021
GTGTAGA206850.012.9996071
AGCATCT1083200.012.9982268
Produced by FastQC (version 0.11.2)