FastQCFastQC Report
Fri 9 Feb 2018
H2LMFBGX5_n01_atac_whole_root_n7_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2LMFBGX5_n01_atac_whole_root_n7_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85756217
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG202950.022.4032045
CCGTACT1918750.021.70170669
ACCGTAC2834900.021.14706269
GACCGTA3836450.019.14687369
CGTACTA1298200.017.97939169
ATACCGC170800.017.895056
GTCGCGC75800.016.1548988
GCACGCC274850.016.00398430
AGACCGT5069850.015.72824269
TCGCGCG84350.015.2535199
TAGACCG184750.014.9565645
TAGGCGG313100.014.6979774
TACCCCG128550.014.6790885
CTAGTAC276300.014.4957173
TAGTACT335200.014.3670044
GTACTAG1120750.014.15706369
ATACCGT151650.014.08105856
TACCGCC198950.013.9572077
TAATACT161700.013.9311814
ATGGACT742150.013.7310464
Produced by FastQC (version 0.11.2)