FastQCFastQC Report
Fri 9 Feb 2018
H2LMFBGX5_n01_atac_whole_root_col-0_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2LMFBGX5_n01_atac_whole_root_col-0_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68923062
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG166600.022.4455555
CAGGCAG1417850.020.24217469
ACAGGCA2010750.020.17025469
GACAGGC2726100.018.46734469
TCGCGCG70000.018.1340569
GTCGCGC67050.017.4913678
ATACCGC145500.016.9757736
TAGGCGG230000.016.2583894
GCACGCC213350.016.04106530
AGACAGG3589950.015.83356369
AGGCAGA1116950.015.24663969
ATACCGT121350.014.867626
CTAGTAC221050.014.6851923
TAGTACT283950.014.2019744
AGTACTA263900.013.9215095
GGTCGCG90100.013.7823497
GCGGATC256450.013.7465517
TAGACCG144800.013.7462215
GGCAGAA838800.013.62550569
AGGTCGC94800.013.5731326
Produced by FastQC (version 0.11.2)