FastQCFastQC Report
Thu 8 Feb 2018
H2LMCBGX5_n01_nlp7_r2_chip_biotin_30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2LMCBGX5_n01_nlp7_r2_chip_biotin_30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38489216
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG622940.1618479316388258No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG387650.10071652277874406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG163500.030.2537231
AGGGGGG116650.029.0384981
TATGCCG132300.024.7652946
CGTATGC137050.024.0326644
GTATGCC143900.022.72084645
ATGCCGT162500.020.35368247
GCCGTCT163800.020.12943649
CTCGTAT162850.019.97013342
TCGTATG173750.019.0752143
TGCCGTC192550.017.35633948
AGTCACG193900.017.33938628
TCTCGTA188850.017.14763541
CGATCAG209100.015.83900433
GTCACGA214950.015.59400729
CCGTCTT236800.014.41973650
CCAGTCA243850.014.1121726
CAGTCAC246900.013.81218427
CACGATC249400.013.5658931
ATCTCGT237600.013.51274540
TCACGAT256300.013.26726730
Produced by FastQC (version 0.11.2)