FastQCFastQC Report
Thu 8 Feb 2018
H2LMCBGX5_n01_nlp7_r1_input_biotin_30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2LMCBGX5_n01_nlp7_r1_input_biotin_30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28076058
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG8586153.05817504722351RNA PCR Primer, Index 17 (100% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGG983160.3501773646428569RNA PCR Primer, Index 17 (100% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGG617510.21994184511230172RNA PCR Primer, Index 17 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT1380700.065.0666649
TATGCCG1394700.065.0464446
CGTATGC1397650.065.00738544
ATGCCGT1393500.064.98295647
GTATGCC1397850.064.9517445
TGCCGTC1392600.064.8827148
CCGTCTT1388350.064.7427250
TCTCGTA1368450.064.7124941
ATCTCGT1355500.064.58816540
TGCTTGA1375350.064.3630258
GCTTGAA1382050.064.3543559
CTTGAAA1397100.063.8190760
ACGTAGA1457450.062.67709432
GTCACGT1480550.061.6251829
CTGCTTG1471750.060.00996457
TCGTATG1514600.060.0071743
CTCGTAT1487900.059.9718842
TTCTGCT1470600.059.9051155
TGAAAAA1523450.059.71249462
TCTGCTT1486250.059.59648556
Produced by FastQC (version 0.11.2)