FastQCFastQC Report
Thu 8 Feb 2018
H2LMCBGX5_n01_nlp7_r1_2xchip_biotin_30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2LMCBGX5_n01_nlp7_r1_2xchip_biotin_30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48808511
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG110400.026.4008751
AGGGGGG102850.020.6588951
TATGCCG101950.018.32159646
CGTATGC108100.017.18337844
GTATGCC122000.015.81995645
ATGCCGT131250.013.59992547
GCCGTCT127700.012.8696249
CTCGTAT142200.012.28516642
TCGTATG150050.011.91873443
GTCACAC233550.011.36932829
TGCCGTC166450.010.78597648
TCTCGTA181850.09.81535541
CCAGTCA273450.09.72200526
CAGTCAC274300.09.64160627
TCCAGTC291850.09.32172525
CACACTT307050.08.8621331
AGTCACA320200.08.44302728
CTCCAGT326600.08.37212224
CCGTCTT217700.08.26282550
ACTCCAG331450.08.19755723
Produced by FastQC (version 0.11.2)