FastQCFastQC Report
Thu 8 Feb 2018
H2LMCBGX5_n01_ev_r2_chip_biotin_30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2LMCBGX5_n01_ev_r2_chip_biotin_30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34274973
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG830680.242357594271482No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG532880.1554720407803093No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG446640.13031082475250963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG191850.033.2812461
AGGGGGG144300.032.7737461
TATGCCG124450.030.84763148
CGTATGC128800.029.86028546
GTATGCC134250.028.5702247
GCCGTCT157000.024.62802951
ATGCCGT159750.024.33458949
TCGTATG167750.023.2150345
CTCGTAT166550.022.98831244
TCTCGTA178700.021.34759743
CCGTCTT202500.019.23048852
TGCCGTC208550.018.6736550
CACAGTC213350.018.58963231
ATCTCGT215050.017.78705242
GTCACAG224900.017.52621729
CAGTCAA234100.017.07929433
CAGTCAC246100.016.82811727
TCACAGT237550.016.68052530
AGTCAAC241400.016.6391734
CCAGTCA253150.016.49568626
Produced by FastQC (version 0.11.2)