FastQCFastQC Report
Thu 8 Feb 2018
H2LMCBGX5_n01_ev_r1_2xchip_biotin_30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2LMCBGX5_n01_ev_r1_2xchip_biotin_30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46084464
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG512990.11131517120390073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG139650.027.7616481
TATGCCG112000.026.11133246
CGTATGC115450.025.2708244
GTATGCC123550.023.8098845
AGGGGGG128200.022.5732351
GCCGTCT128850.019.77584649
ATGCCGT148300.019.13756847
CTCGTAT154950.017.8698542
TCGTATG159500.017.07887343
CACTAGC240650.016.02216331
ACTAGCT246150.015.776710532
TAGCTTA250350.015.71429734
TATCTCG189900.015.48792939
TCTCGTA180000.015.40214441
GTCACTA257250.015.17405229
TGCCGTC192600.014.44871348
TCACTAG273700.014.30056830
CCGTCTT198650.013.73098550
AGTCACT291250.013.60334728
CAGTCAC305000.012.95572927
Produced by FastQC (version 0.11.2)