FastQCFastQC Report
Thu 8 Feb 2018
H2LMCBGX5_n01_bzip3_r2_input_biotin_30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2LMCBGX5_n01_bzip3_r2_input_biotin_30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26696048
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA7838252.9361087453843355TruSeq Adapter, Index 20 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG1954530.7321420758608166TruSeq Adapter, Index 20 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAT979420.3668782735182376TruSeq Adapter, Index 20 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA575090.2154213987029091TruSeq Adapter, Index 20 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAC538180.20159538220788334TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT1551300.062.44141449
TGCCGTC1542900.062.30285650
CGTATGC1570200.062.2767746
TATGCCG1569100.062.23565348
GTATGCC1575650.062.18821347
GCCGTCT1517900.062.10035751
CGTGGCC1644750.061.3920433
TCGTATG1581550.061.2661845
GGCCTTA1643600.061.1451636
CTCGTAT1567200.061.0205544
GCCTTAT1634450.061.01432437
GTGGCCT1654650.060.9081534
TATCTCG1582650.060.49664341
ACGTGGC1673150.060.46779632
CCGTCTT1569150.060.27197352
TCTCGTA1581850.060.18757243
TCACGTG1685700.060.161930
GTCACGT1688300.060.10762829
CACGTGG1687300.060.0725831
ATCTCGT1595200.059.91066442
Produced by FastQC (version 0.11.2)