FastQCFastQC Report
Thu 8 Feb 2018
H2LMCBGX5_n01_bzip3_r2_2xchip_biotin_30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2LMCBGX5_n01_bzip3_r2_2xchip_biotin_30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37893509
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG97900.030.1862148
CGTATGC104900.028.43629546
TGGGGGG91000.026.2303961
GTATGCC115300.026.14046747
ATGCCGT112750.024.95578449
TGCCGTC119000.023.87717850
CTCGTAT138850.022.626944
GCCGTCT118350.021.76100251
TCGTATG141600.021.11483445
AGGGGGG78900.019.5857941
CAGTCAC243250.019.29305527
TCTCGTA166550.019.13272743
CCAGTCA257500.018.39944326
GTCACAT258150.018.1137829
TCCAGTC262450.017.94716525
ATGTCAG263750.017.6633734
TGTCAGA268700.017.49301335
GTCAGAA273100.017.1353836
ACTCCAG274150.017.13084823
CTCCAGT285200.016.61226824
Produced by FastQC (version 0.11.2)