FastQCFastQC Report
Thu 8 Feb 2018
H2LMCBGX5_n01_bzip3_r1_chip_biotin_30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2LMCBGX5_n01_bzip3_r1_chip_biotin_30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45302019
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG489330.10801505336881342No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG470810.10392693535358766TruSeq Adapter, Index 5 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG183550.037.7319446
CGTATGC193700.035.73658444
GTATGCC204250.034.49967645
ATGCCGT203650.033.0074747
TGCCGTC209150.031.94148
GCCGTCT206800.030.85249349
TGGGGGG132100.029.7940961
TCGTATG227050.028.79885543
CTCGTAT187550.027.16953742
GTCACAC310250.025.7096229
CACAGTG318150.025.18984833
AGGGGGG100950.024.431331
ACACAGT343700.023.6117932
CAGTCAC340000.023.55981827
TCTCGTA221450.022.83878141
CCAGTCA352750.022.63983226
CCGTCTT289300.022.46013850
TCCAGTC359100.022.40270625
CACACAG361150.022.3649831
ACTCCAG371600.021.79744523
Produced by FastQC (version 0.11.2)