FastQCFastQC Report
Thu 8 Feb 2018
H2LMCBGX5_n01_bzip1_r2_2xchip_biotin_30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2LMCBGX5_n01_bzip1_r2_2xchip_biotin_30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36510858
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG90150.027.0127751
TATGCCG101950.024.75539448
CGTATGC107700.023.69073946
GTATGCC118200.021.67371447
ATGCCGT119150.021.06639749
TGCCGTC119750.020.90323650
CCGTATC135700.019.94224738
GCCGTCT123000.019.64851
GTTCCGT144300.019.1089535
CTCGTAT136800.018.2471244
AGGGGGG79150.018.2161061
TTCCGTA151600.018.18889436
CGTATCT148250.017.32362639
TCGTATG145700.017.22752845
AGTTCCG165350.016.75890534
TCTCGTA160650.015.77413543
TATCTCG176900.014.48107941
TCCGTAT196050.014.13588137
GTCACAG205700.013.84751629
CACAGTT211900.013.62306231
Produced by FastQC (version 0.11.2)