FastQCFastQC Report
Thu 8 Feb 2018
H2LMCBGX5_n01_bzip1_r1_input_biotin_30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2LMCBGX5_n01_bzip1_r1_input_biotin_30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36848212
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG3866341.049261223312545TruSeq Adapter, Index 6 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGG501740.136164001661736TruSeq Adapter, Index 6 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT656750.062.95046649
GTATGCC666650.062.91673345
TATGCCG665300.062.91518846
CGTATGC667750.062.82834644
ATGCCGT667150.062.62550747
TGCCGTC666200.062.51755548
TCTCGTA670100.062.02869841
CCGTCTT667700.061.92378650
ATCTCGT676450.061.53251340
GCTTGAA665150.061.21896459
TGCTTGA662450.061.16644758
CTTGAAA685100.059.66269760
GCCAATA715450.059.07698434
CAATATC709100.058.99164636
CCAATAT718050.058.8713135
CGCCAAT720450.058.731533
AATATCT711650.058.66658837
CACGCCA738800.057.3533731
TCACGCC747550.056.6285430
TATCTCG772200.053.96910539
Produced by FastQC (version 0.11.2)