FastQCFastQC Report
Thu 8 Feb 2018
H2LMCBGX5_n01_bzip1_r1_chip_biotin_30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2LMCBGX5_n01_bzip1_r1_chip_biotin_30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44227579
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG470080.10628662265235002TruSeq Adapter, Index 4 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG161250.031.367446
CGTATGC168500.030.22211644
GTATGCC178700.028.45868745
ATGCCGT180900.028.11262347
TGCCGTC182750.027.73332248
TGGGGGG84750.026.3320751
GCCGTCT194600.026.09857649
CTCGTAT195000.025.1578842
TCGTATG213800.024.07639143
ACTGACC229700.022.43191732
TCTCGTA224700.021.57141141
AGGGGGG93700.019.5467871
CCGTCTT269100.019.10418550
CTGACCA285800.018.19081133
CACTGAC285950.018.07876231
GTCACTG298200.017.48491329
ATCTCGT281950.017.23996240
CAGTCAC302450.017.23812527
AGTCACT301000.017.21849328
ACTCCAG322950.016.26121523
Produced by FastQC (version 0.11.2)