FastQCFastQC Report
Fri 16 Feb 2018
H2LHHBGX5_n02_at11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2LHHBGX5_n02_at11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29268973
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT792520.27077137281174846No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC671030.22926325430003983No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA616560.2106531035441524No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA540780.18476220535650498No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC522620.17855768290879218No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG481470.16449842637116102No Hit
TAAACAGTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAA471250.1610066742006971No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT430000.1469132517905565No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC423880.1448223003929793No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC422480.14434397817784725No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA407810.1393318446807136No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG406530.13889452151259288No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT387550.13240983891030272No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG381230.13025055576770664No Hit
CGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACT375530.12830310103466902No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA362870.12397770157497497No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTT356110.12166808859333739No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTC345960.11820025253363006No Hit
CAGCTCTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTT344080.11755793413045276No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG330110.11278496174088512No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT325660.11126458041421541No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG321400.10980911424531363No Hit
GATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATA316540.10814865284135525No Hit
GGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAG311630.10647110850114214No Hit
AAACAGTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAAT311060.1062763630278384No Hit
GCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAG301530.10302035537768954No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCAAT61900.069.3980941
CTTGTTA125800.060.801811
GTTACGA143850.052.8292354
TTACGAC148450.051.2984775
GAGTCTA114750.050.7942732
GGAGTCT116250.050.0748251
CCTGGCC285300.047.9095575
GGGCACT19200.045.3469431
AGTCTAA87400.044.6249473
TGGCCCG320750.042.70717
CAAAGGA113850.041.3609738
ATCAAAG122200.040.9379356
ACGACTT187450.040.5898557
TCAAAGG132500.040.52767
GCTTGTA117500.039.6364147
TCTAACG99400.039.1026655
GGTCTGG69100.039.0519833
TGTTACG196850.038.8220943
CCGGCAT42250.037.9788671
CTGGCCC363400.037.8717466
Produced by FastQC (version 0.11.2)