FastQCFastQC Report
Fri 16 Feb 2018
H2LHHBGX5_n02_ac11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2LHHBGX5_n02_ac11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50775520
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2388980.4704983818974183No Hit
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT1241860.24457848979193123No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA1223340.24093106284288177No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATC1144740.22545116229238027Illumina Single End PCR Primer 1 (96% over 33bp)
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC959520.18897295389589314No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT904190.17807597046765844No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA896620.17658509454949944No Hit
GCGAGCGCTTGTAATCCAAGTGACTGGGAGGCATGTGGTGGCGGATGGAG839050.16524695365010542No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC808770.15928344997746946No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG747300.14717722240953907No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG721920.1421787507050642No Hit
GGCGGATGGAGTGAGAGGAGGAGTCCTGGCTGATGCTTGGCGGTGGCGAC690500.13599072939085607No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG688650.13562638058655038No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC640310.1261060448026923No Hit
CTTGGCTCATGGATCGATGAAGAACGCAGCTAGCTGCGATAAATAGTGCG636630.12538128610007343No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT626930.12347091669371382No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTT599650.11809824891995198No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC586880.11558325744374454No Hit
GTCCAATCAAAGGATAAGTCTCAACAGATCGCAGTATGGAGGCTGCTCTA535060.10537755201719254No Hit
CTCAACCAGACGTACCATCGGGAATCAACCCAATGGTGCAATGTGCATTC533990.10516682054659411No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTC532000.10477489940034096No Hit
GGCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAG521760.10275817953218401No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGAG1013000.0104.5430453
GGGAGAG1965250.0102.486965
GGAGAGA889450.098.693296
GGGGAGA2726150.091.940334
GGTAGGA214100.085.532911
GAAAACG189050.084.226317
GAGAGAG1065400.080.599197
GTAGGAA239750.075.574922
AGAAAGG488650.075.561018
AAAGAGG1845850.075.2733158
GTCCAAT118900.074.981071
GAGAAAG408350.073.879597
GTGGGGA3751700.073.80532
AAGCGGG536900.072.932689
GTGGGAA1519700.070.755552
AAAGCGG654050.070.708998
AGAGAGG1458150.068.7163248
GGAAAGG710400.067.787646
TAGGAAA242700.066.7703863
AGAGCGG576850.066.56668
Produced by FastQC (version 0.11.2)