FastQCFastQC Report
Fri 16 Feb 2018
H2LHHBGX5_n01_bc11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2LHHBGX5_n01_bc11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30873797
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCACCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCAT1552470.5028438840872084No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA1233150.39941637240148986No Hit
CTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGTTTTGG1146630.37139260843102645No Hit
CAGCTTTGCAACCATACTTCCCCCGGAACCGAAAAACTTTCGTTTCCGGG838560.2716089634196921No Hit
CAGAAATCAGAGACAGTCAACGCCCGCAAGGGCCATCTGTCTCCTCTGTT792190.2565897547360307No Hit
GTTAGTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAG763420.24727117302740573No Hit
GTGATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAA719570.23306819047880634No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCAC695390.22523630637333011No Hit
GTCGTCTGCAAGTGATCTTGCGCCCCGACATTGTACCAGAGTAATCCGGT673410.2181170006397334No Hit
TGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTTT634980.20566955208003732No Hit
CTTGTTACGACTTTTGCCCGGTTCAAGCCACTGCGATTAAAGCAGTTTCC632930.20500555859714956No Hit
CCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTATTCTA630690.2042800242548722No Hit
CAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGGAATTCCT610760.19782471200416327No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTCAGCTTTG603860.19558980711054103No Hit
CTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAGTTACTG589940.1910811294121031No Hit
CAGCTCTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTT570790.18487845858415147No Hit
CGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACT559790.18131556672475369No Hit
CCCGTCCGTCCCTCTTAACCATTATCTCAGATCAGAAAACCAACAAAATA556620.18028880607072723No Hit
GTCGGCGGTTATATGCTTGTCTCAAAGGCTAAGCCATGCATGTCTAAGTT544820.17646679480337324No Hit
CTAGCATATACGCAAGTTATTGGGTAGTAAACCTAATTTAAAAGATAAAA511360.16562912556560505No Hit
CGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACCTGTTATTGCTC497600.16117227174875834No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTTATTCCGATAACGAGC490660.15892441088473827No Hit
GTTTTACCCTACTGTGATATGTTGTTGCGATAGTAATCCTGCTCAGTACG455780.1476268046978478No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTTC452850.14667777986620822No Hit
CTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATTC436360.141336681069711No Hit
TAAACAGTCAACTTAGAACTGGCACGGACTCGGGGAATCCGACTGTCTAA421700.13658831791891357No Hit
CAAATATTCAAGTGAGATCCTTGAAGACTGAAGTGGAGAAGGGTTCCACG419530.13588545652483236No Hit
CCTGGCCCGGACACCGTGAGGATTGACAGATTGAGAGCTCTTTCATGATT417440.13520850707154677No Hit
CAAACGTTGAGTTAAAGTGCCAAACTTTCCGCTCATGAGACCCCATGAAA416020.13474857012242453No Hit
GACAGATTGAGAGCTCTTTCATGATTCGGTGGTTGGTGGTGCATGGCCGT401980.13020102451279317No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT392130.12701061680233242No Hit
CTGGAAATCAAGATCAAGCGAGCTTTTGCCCTTTTACTCTACGAGAGGTT383190.12411495741842184No Hit
GATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATA382050.12374571226208425No Hit
GCCTTGTCTAGGTGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCTC381420.12354165572831875No Hit
CAAAGGGCAGGGACGTAATCAACGTGAGCTGATGACTCACACTTACTAGG380360.12319832251277676No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTTCTTTAAGTTT373740.12105410941193918No Hit
CGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGGTA370570.12002734875791274No Hit
CTCGTTAACAGGTTTAAATTGTACCCATTCCGATAACCGGCCTCAAAGAG370450.11998848084671931No Hit
ATTTGTCTGGTTTATTCCGATAACGAGCGAGACTCTAGCCTACTAAATAG367150.11891961328889997No Hit
CGGAAACCTCTCGTAGAGTAAAAGGGCAAAAGCTCGCTTGATCTTGATTT356260.11539235034809617No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT352050.11402873446372665TruSeq Adapter, Index 6 (97% over 36bp)
CCCCAGTCAAACTCCCCGCCTGACACTGTCTTCGGTGCGAGTCGTTAAAT351530.1138603068485551No Hit
CTCCAGTGTAGCGCGCGTGCAGCCCTGGACATCTAAGGGCATCACAGACC350460.11351373464041367No Hit
GCCAGTTTTCCCATACGTTACCTTAATTGGTTGCATATGGTGGCTAGCAA347230.11246754003079051No Hit
GTGTAATCCATCTGATAAATCGAAAGGGAAACGGGTTAATATTCCCGTAC333690.10808194405113178No Hit
CACGAGTATTCAGGCATTAGCCCGCATTGAGCACTCTAATTTTTTCAAGG333670.10807546606593287No Hit
CTTGATTAATGAAGACATTCTTGGCAAATGCTTTCGCAGTCGTACGTCTC332820.1078001516949794No Hit
ATTAGATTAAAACCAATCGGATTATTAATTTTAATCCGTTAATTGGTGAC332550.10771269889479418No Hit
CGAGAATTGAAATATTATATGTATTTCGAAATTTTTGATTTAAGTTAAAT323310.10471986973290004No Hit
GTCGATCCTAAGATATTGGCTAGTGCCGTTTTTTAAAATAAAATAGAGGA314790.10196024803816647No Hit
CCAGCTCTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGT309430.10022414800485993No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTTA147750.071.581241
ACCTGGC365900.068.915014
CACCTGG372350.067.663923
CCTGGCC389850.064.865045
GTTACGA164200.064.249984
TTACGAC164550.064.068755
GTCCAAT78100.063.9754451
GGGCACT16200.062.662681
TCCAATC81450.060.4352762
CCGGCAT46050.057.0647771
GCCCGGA452250.056.1689539
CTGGCCC451350.056.0583766
TGGCCCG457650.055.3803987
GGCCGAC37250.055.0837481
GGCGGTT176300.051.9622844
ACGACTT207650.050.735197
GGAGTCT85950.050.2585831
GGTTATA182900.050.2038657
GAGTCTA87450.049.4584122
ATCGAAG500700.047.342496
Produced by FastQC (version 0.11.2)