FastQCFastQC Report
Wed 16 May 2018
H2L3KAFXY_n01_ah8921i-0418.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L3KAFXY_n01_ah8921i-0418.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5054739
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT1036932.0514016648535165TruSeq Adapter, Index 19 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG98830.1955194917086718No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGCTCTCGTAT80530.15931584202468219TruSeq Adapter, Index 19 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGAGCTCGTAT62420.12348807722812197TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG164450.042.3653511
AGTCACG163350.042.23357428
CACACGT165450.042.2160812
ACACGTC165750.042.15294313
ACGTCTG166200.042.05204415
GTCACGT163750.042.0099129
CGTCTGA166400.041.8292216
GATCGGA157800.041.786661
CACGTCT167050.041.77222414
GAGCACA167150.041.759579
CACGTGA164850.041.75710731
AGCACAC167250.041.73501210
TCACGTG165150.041.69416430
CGATCTC126850.041.536440
GAACTCC165950.041.47826821
CAGTCAC165750.041.4627827
CTCGTAT145750.041.40333644
GATCTCG128100.041.32040841
TCGGAAG170100.041.3196033
GTCTGAA168350.041.2789717
Produced by FastQC (version 0.11.2)