FastQCFastQC Report
Wed 16 May 2018
H2L3KAFXY_n01_ah8921a-0418.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L3KAFXY_n01_ah8921a-0418.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11047463
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT345270.31253329384312034TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG58000.038.76468732
CGTCCGC58300.038.48974233
GCACACG61500.038.09605811
ACACGTC64900.036.16806413
CACGTCC62850.035.9133131
GTCCGCA61300.035.70886234
ACGTCTG65950.035.692315
AGTCACG64150.035.63134828
CACACGT65800.035.60649512
CCGCACA57050.035.36088636
GTCACGT65150.035.11836229
GATCGGA67050.034.758891
CACGTCT67950.034.70650514
AGCACAC68150.034.57221210
TCACGTC67050.034.09040530
CGTCTGA69000.034.0827116
TCCGCAC64350.034.01636535
CAGTCAC69100.033.01506427
CATCTCG54850.032.92880641
GAGCACA72200.032.6633839
Produced by FastQC (version 0.11.2)