FastQCFastQC Report
Wed 16 May 2018
H2L3KAFXY_n01_ah8869b-0418.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L3KAFXY_n01_ah8869b-0418.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9572464
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT1265371.3218853578347225TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGCTCTCGTAT149790.15648008704968752TruSeq Adapter, Index 16 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGAGCTCGTAT142090.14843618111282528TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG223450.042.4635735
GTCCCGA200550.042.33207336
GCACACG238500.041.95894611
GATCGGA238350.041.771631
ACCCGTC226050.041.66394432
CCCGTCC228400.041.65886733
ACGTCTG240850.041.6328415
CCGTCCC228500.041.4769634
CACGTCT242350.041.37515614
TCACCCG227750.041.3716230
ACACGTC242550.041.36824813
CACCCGT228150.041.2508831
CCCGATC164150.041.21159438
GTCACCC230650.041.0608629
CGTCTGA243650.041.05507716
CACACGT244200.040.99864612
AGCACAC244650.040.95813410
TCCCGAT184900.040.89361637
GAGCACA246400.040.7833029
CAGTCAC236850.040.6638527
Produced by FastQC (version 0.11.2)