FastQCFastQC Report
Wed 16 May 2018
H2L3KAFXY_n01_ah8869a-0418.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L3KAFXY_n01_ah8869a-0418.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6076929
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT176800.29093642528981334TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG30200.036.34912511
ACACGTC31700.034.62941713
CACACGT32050.034.45688612
ACGTCTG32550.034.4009715
GATCGGA31150.034.335751
CACGTCT33250.033.6767414
CGTCTGA33350.033.5095216
CACAGTC33000.033.4660531
CAGTCAC33900.032.77171327
GAGCACA34900.032.084849
AGCACAC35350.031.67588210
TCCAGTC35550.031.56007825
AGTCACA35100.031.52596528
CCAGTCA35700.031.18098326
GTCACAG35650.031.16326129
AGAGCAC36700.030.4518
TCGGAAG37450.030.4356043
GAACTCC36400.030.39928421
CTCGTAT32400.030.2152444
ACTCCAG38350.029.19773723
Produced by FastQC (version 0.11.2)