FastQCFastQC Report
Wed 16 May 2018
H2L3KAFXY_n01_ah8868b-0418.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L3KAFXY_n01_ah8868b-0418.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8105382
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT1953202.4097568751232203TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCGCGTAT81450.10048878633974316TruSeq Adapter, Index 14 (97% over 45bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG284700.042.4526211
GATCGGA271000.042.275451
ACGTCTG286500.042.2015215
ACACGTC286450.042.19326413
CACGTCT287500.042.0774314
CACACGT287300.042.03780712
AGCACAC288250.042.00584810
CGTCTGA288400.041.9311216
AGAGCAC291750.041.660788
GAGCACA290400.041.6574949
TCGGAAG292650.041.6533853
GAACTCC287400.041.64810621
AGTTCCG280700.041.60088334
TCCAGTC287650.041.52116425
CAGTCAC287900.041.43187327
CCGTATC254900.041.39317338
GTTCCGT279750.041.30963535
CCAGTCA288350.041.2756626
GTCACAG288950.041.22827529
CTCGTAT249650.041.22380444
Produced by FastQC (version 0.11.2)