FastQCFastQC Report
Wed 16 May 2018
H2L3KAFXY_n01_ah8868a-0418.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L3KAFXY_n01_ah8868a-0418.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5656859
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT341660.6039747499451551TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG61550.040.63786311
CACACGT63050.039.70665412
GATCGGA59350.039.526861
CGTCTGA63700.039.0597316
ACGTCTG64250.038.9992915
ACACGTC64800.038.80407313
CACGTCT64700.038.66003814
AGCACAC65150.038.52739710
GAGCACA66450.038.00549
TCCAGTC64550.037.6933725
CAGTCAC65300.037.59833527
TCGGAAG66650.037.570593
CCAGTCA65800.037.07793826
AGAGCAC68100.037.052268
GAACTCC66350.036.96854421
CACATGT67300.036.5790731
AGTCACA67500.036.53587328
GTCACAT67650.036.4873829
CTCCAGT67250.036.24513624
TCACATG68600.036.07829730
Produced by FastQC (version 0.11.2)