FastQCFastQC Report
Wed 16 May 2018
H2L3KAFXY_n01_ah8867b-0418.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L3KAFXY_n01_ah8867b-0418.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4100954
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC358720.8747232960915924TruSeq Adapter, Index 12 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56820.13855312690656857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA54350.041.13881
ACACGTC57700.040.987613
GCACACG58200.040.7095811
CACGTCT58200.040.55986414
ACGTCTG58500.040.31426215
CGTCTGA58600.040.1703816
CACACGT58900.040.15253412
CGTATGC55900.039.86589444
AGCACAC59250.039.8396310
CAGTCAC59000.039.78520627
GAGCACA60300.039.219359
GAACTCC60450.038.9395921
GTCACCT59700.038.8396729
TCGTATG56250.038.83565543
AGAGCAC60900.038.8329478
AGTCACC60350.038.67651428
ATCTCGT48600.038.65676540
TCCAGTC61650.038.431925
TCGGAAG62300.038.3923
CCAGTCA61150.038.24247426
Produced by FastQC (version 0.11.2)