FastQCFastQC Report
Wed 16 May 2018
H2L3KAFXY_n01_ah8867a-0418.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L3KAFXY_n01_ah8867a-0418.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4273214
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC967772.2647356299029258TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATGC72480.1696147209103031TruSeq Adapter, Index 7 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57210.13388049369865399No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAGCTCGTATGC46990.10996406919943631TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATGTCGTATGC45040.10540075924117069TruSeq Adapter, Index 7 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA162550.042.8770871
GCACACG170750.042.70948811
ACACGTC171900.042.5901313
CACACGT171800.042.4612612
AGCACAC172750.042.316910
ACGTCTG172500.042.3144615
CGTCTGA172350.042.27470416
GAGCACA173250.042.2582639
CACGTCT173050.042.25625214
AGAGCAC174900.041.97288
TCGGAAG175200.041.934873
CGTATGC148600.041.85223844
TCCAGTC172150.041.8141325
CAGTCAC172100.041.6979627
CCAGTCA171600.041.6532826
GAACTCC172800.041.63040521
CATCTCG125950.041.55353539
ACTCCAG173250.041.4968823
AGTCACC172500.041.34668428
TCTGAAC176500.041.30563718
Produced by FastQC (version 0.11.2)