FastQCFastQC Report
Wed 16 May 2018
H2L3KAFXY_n01_ah8643i-0418.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L3KAFXY_n01_ah8643i-0418.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6094187
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1113561.8272494756068365TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG147600.042.18026411
GTCACGC143600.042.1449329
ACACGTC147550.042.0901913
ACGTCTG148350.041.89252515
GATCGGA143050.041.8614121
AGTCACG145500.041.80592328
CACACGT148400.041.80463412
TCACGCC143800.041.67360330
CACGCCA144300.041.51395831
CACGTCT150000.041.50504314
AGCACAC150450.041.36661510
CGTCTGA150250.041.36277416
CGTATGC143000.041.2759844
TCGGAAG153300.040.9340133
GAGCACA152150.040.87559
AGAGCAC152650.040.8569078
TATCTCG137800.040.8053739
TCCAGTC149300.040.80148725
CAGTCAC149350.040.71350527
CCAGTCA149300.040.56572726
Produced by FastQC (version 0.11.2)