FastQCFastQC Report
Wed 16 May 2018
H2L3KAFXY_n01_ah8643a-0418.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L3KAFXY_n01_ah8643a-0418.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5440594
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC241560.44399563724108065TruSeq Adapter, Index 5 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG86430.15886133021504636No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG50150.040.09431511
ACGTCTG51200.039.18577615
ACACGTC51550.039.04846213
GTCACAC51400.038.6488129
GATCGGA48600.038.491591
CACACGT52400.038.45702712
CACGTCT52600.038.4355714
CGTCTGA52700.038.15356416
CACACAG52500.037.630231
CGTATGC48000.037.44521344
AGCACAC54100.037.12624410
GAACTCC53800.036.92334421
CAGTCAC53550.036.72667327
AGTCACA54400.036.51677328
GAGCACA55350.036.407049
CCAGTCA54450.036.07921626
TCCAGTC54700.035.99475525
CACAGTG55000.035.95973633
AGAGCAC56550.035.7508548
TCGGAAG56850.035.570373
Produced by FastQC (version 0.11.2)