FastQCFastQC Report
Wed 16 May 2018
H2L3KAFXY_n01_ah8630i-0418.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L3KAFXY_n01_ah8630i-0418.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7114221
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC757051.064136185817112TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG116900.041.83373311
ACACGTC117450.041.58222213
ACGTCTG118100.041.37199415
CACGTCT118750.041.14553514
CACACGT118800.041.1094112
AGCACAC120700.040.58958410
GAACTCC118650.040.51215721
CGTCTGA120750.040.48225416
CAGTCAC119850.040.2177927
GAGCACA122300.040.167069
ACTGACC115600.040.13614732
CGTATGC116750.040.0979844
GATCGGA116800.040.02011
TCCAGTC121200.039.896625
CCAGTCA120900.039.7957226
AGTCACT122350.039.503928
ACTCCAG122700.039.3183923
TCGGAAG125550.039.2933
GTCTGAA124200.039.14519517
AGAGCAC126400.038.96868
Produced by FastQC (version 0.11.2)