FastQCFastQC Report
Wed 16 May 2018
H2L3KAFXY_n01_ah8630a-0418.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L3KAFXY_n01_ah8630a-0418.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5768348
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC118540.20550077769233063TruSeq Adapter, Index 2 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG68280.11837011220543558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC20850.033.86919813
GCACACG21100.033.67613211
CACGTCT22050.032.22551314
ACGTCTG22700.031.30275515
CACACGT22750.031.23395712
GATCGGA22600.029.8923511
AGCACAC23650.029.67301610
CCGATGT23900.029.27159733
CGTCTGA24650.029.00496516
GTCACCG24750.028.62161329
TCCAGTC24850.028.59471725
CAGTCAC24800.028.56366227
CGTATGC22550.028.38963944
TATCTCG21100.028.35958739
CACCGAT24900.028.18389531
GAACTCC25450.028.17965921
CCAGTCA25200.028.02296826
AGTCACC25350.027.94393728
ACCGATG25250.027.7065832
GAGCACA25900.027.3500589
Produced by FastQC (version 0.11.2)