FastQCFastQC Report
Wed 16 May 2018
H2L3KAFXY_n01_ah8189b-0418.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L3KAFXY_n01_ah8189b-0418.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5630862
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT153350.2723384092879563TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC25300.036.6945213
GCACACG25200.036.4029911
CACACGT26700.034.5232812
CACGTCT26850.034.00267414
ACGTCTG26950.033.87650315
CGTCTGA27350.033.38046316
GAACTCC27050.032.93681721
AGCACAC28200.032.76436210
GATCGGA25950.032.313351
CAGTCAC28550.031.59243827
GAGCACA29200.031.1905429
TCCAGTC29100.030.84385925
CCAGTCA29550.030.67194226
AGAGCAC30350.030.2980928
TATCTCG25950.030.2649641
TCGGAAG30700.029.888983
CTCGTAT27250.029.46671944
GTCACAT30850.029.37996929
AGTCACA30700.029.23662428
TCTCGTA27250.028.82087343
Produced by FastQC (version 0.11.2)