FastQCFastQC Report
Wed 16 May 2018
H2L3KAFXY_n01_ah8189a-0418.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2L3KAFXY_n01_ah8189a-0418.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6113900
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT72080.11789528778684637TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG13700.029.06391711
ACACGTC14750.026.9949613
CACACGT15500.026.11453212
ACGTCTG15850.025.12149215
CGTCTGA16100.025.00468316
GATCGGA14550.024.957951
CACGTCT16350.024.62234914
GTCACAC15550.024.47558829
AGCACAC17150.023.47378310
CACACTG17550.022.43884331
CAGTCAC17400.022.2524227
TATCTCG17200.022.12709641
GAACTCC18900.021.30045721
TCCAGTC18450.021.22415425
GAGCACA19650.021.0474039
CTCGTAT18500.020.8100544
CCAGTCA18850.020.42365526
TCGGAAG20200.020.2611583
AGTCACA19250.020.11387828
AGAGCAC20850.019.5195088
Produced by FastQC (version 0.11.2)